CD147 RNA干扰对前列腺癌LNCaP-AI细胞体外侵袭力的影响

doi:10.13481/j.1671-587x.20150305

Abstract

Abstract:

Objective To explore the effect of CD147 on the invasion of prostate cancer LNCaP-AI cells and its possible mechanism in vitro, and to provide basis for research on prostate cancer invasion. Methods The shRNA vector targeting CD147 gene and negative control vector were transfected into LNCaP-AI cells by Lipofectamine 2000, respectively.The stable cell lines were obtained after G418 screening and divided into AI/shCD147 group (experiment group) and AI/Scramble group (control group), respectively.Western blotting method was employed to detect the expression of CD147 protein in the cells in two groups.Transwell invasion assay was used to evaluate the invasion ability of LNCaP-AI cells in vitro.The level of matrix metalloproteinase-2 (MMP-2) was determined by Western blotting method. Results Compared with AI/Scramble group, the CD147 protein in the LNCaP-AI cells in AI/shCD147 group was significantly decreased.The Transwell invasion assay showed that the A value of LNCaP-AI cells in AI/shCD147 group (1.43±0.2) was significantly lower than that in AI/Scramble group (2.08±0.3) (P<0.05).Compared with AI/Scramble group (0.85±0.02), the expression of MMP-2 protein in AI/shCD147 group (0.46±0.08) was significantly decreased (P<0.05). Conclusion CD147 can promote the invasion ability of LNCaP-AI cells in vitro through inducing the MMP-2 secretion.It is demonstrated that targeting CD147 could potentially be a new approach for prostate cancer gene therapy.

Key words: prostate neoplasms, CD147, RNA interference, matrix metalloproteinase, invasion

CLC Number:

R737.25

FANG Fang, ZHANG Wansheng, TANG Huayong, ZHAO Liangzhong, CHEN Shuang, LI Qiang, WANG Liguo. Influence of CD147 RNA interference in invasion of prostate cancer LNCaP-AI cells in vitro[J].Journal of Jilin University Medicine Edition, 2015, 41(03): 460-463.

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Influence of CD147 RNA interference in invasion of prostate cancer LNCaP-AI cells in vitro

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