橙皮素对舌癌细胞的生长抑制作用及其机制

doi:10.13481/j.1671-587x.20150324

Abstract

Abstract:

Objective To detect the influence of hesperetin in the growth of tongue cancer cells and the activation of Notch1 signaling and to explore its mechanism. Methods The Tca8113 cells were cultured and divided into DMSO control and 25, 50, 75, 100, 125 μmol·L^-1 hesperetin groups.Then MTT assay was performed to detect the inhibitory rate of proliferation of Tca8113 cells after treatment with different concentrations of hesperetin.Flow cytometry was used to detect the the percentage of apoptotic cells and the distribution of cell cycle, and qPCR and Western blotting Methods were used respectively to analyze the relative expression levels of Notch1 and Hes-1 mRNA and protein. Results The MTT Results revealed that compared with DMSO control group, the inhibitory rates of proliferation of Tca8113 cells in 125 μmol·L^-1 hesperetin groups at 24, 48, and 72 h(7.88%±1.9%, 26.28%±2.2%, 47.05%±1.9%)were significantly increased(P<0.05).Moreover, after hesperetin treatment for 72 h, the inhibitory rates of proliferation of the Tca8113 cells in 25, 50, 75, 100, and 125 μmol·L^-1 hesperetin groups were (6.14±1.2)%, (28.69±2.1)%, (28.33±2.6)%, (45.26±3.0)%, and (47.05±1.9)%, respectively; hesperetin inhibited the growth of Tca8113 cells in a time- and dose-dependent manner.The flow cytometry Results showed that after hesperetin treatment for 72 h, compared with DMSO control group, the apoptotic rates of the Tca8113 cells in 50 and 100 μmol·L^-1 hesperetin groups were increased significantly from (2.9±0.5)% to (23.4±1.7)% and (35.1±1.9)%(P<0.05);the percentages of the Tca8113 cells in G₀/G₁ phage in 50 and 100 μmol·L^-1 hesperetin groups were increased significantly from (18.6±1.3)% to (33.4±1.5)% and (40.5±1.9)% (P<0.05), and the percentages of the Tca8113 cells in S phage were decreased from (70.3±2.5)% to (56.8±2.0)% and (48.7±1.8)%(P<0.05).The qPCR and Western blotting Results showed that the expression levels of Notch1 and Hes-1 mRNA and protein were all increased compared with DMSO control group(P<0.05). Conclusion Hesperetin can inhibit the growth and induce the apoptosis and G₀/G₁ arrest in the Tca8113 cells, and its mechanism may be related to activating the Notch1 signaling.

Key words: hesperetin, tongue neoplasms, Notch1, Hes-1

CLC Number:

R739.86

LIU Zhihui, YANG Wei. Suppressive effect of hesperetin on growth of tongue cancer cells and its mechanism[J].Journal of Jilin University Medicine Edition, 2015, 41(03): 558-562.

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Suppressive effect of hesperetin on growth of tongue cancer cells and its mechanism

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