补骨脂乙素对舌鳞状细胞癌Tca8113细胞增殖的抑制作用和凋亡诱导作用及其机制

doi:10.13481/j.1671-587x.20160418

Abstract

Abstract:

Objective: To observe the effects of isobavachalcone (IBC) on the proliferation and apoptosis of tongue squamous cell carcinoma Tca-8113 cells,and to explore their mechanisms. Methods: The Tca8113 cells cultured in vitro were divided into control group and different doses (0,10,20,40,80 μmol·L^-1) of IBC groups. The inhibitory rates of cell proliferation were detected by MTT method.The apoptosis was detected by flow cytometry.Western blotting method was used to detect the expressions of Akt,p-Akt,Erk,p-Erk,Bax,Bcl-2 and Caspase-3 proteins in the Tca8113 cells in various groups. Results: The MTT results showed that the inhibitory rates of proliferation of Tca8113 cells were increased in a concentration- and time- dependent manner;the IC₅₀ at 12,24 and 48 h were (285.13±8.97),(132.40±7.76), and (58.56±5.93) μmol·L^-1, respectively;and there were significant differences between different time points (P < 0.05).The FCM results showed that the apoptotic rates of Tca8113 cells in 20 and 40 μmol·L^-1 IBC groups at 48 h were (8.21±2.32)% and (22.45±1.18)%, respectively;compared with control group (1.69%±0.65%),the differences were statistically significant (P < 0.05). The Western blotting results showed that the expression levels of Bcl-2,p-Akt, and p-Erk, in 20 and 40 μmol·L^-1 IBC groups were decreased and the expression levels of Bax were increased compared with control group (P < 0.05);the expression levels of Akt and Erk had no significant changes(P > 0.05).The expression levels of Caspase-3 in Tca8113 cells in 40 μmol·L^-1 IBC group at 48 h were increased compared with control group (P < 0.05). Conclusion: IBC could inhibit the proliferation and induce the apoptosis of Tca8113 cells;Akt and Erk signaling pathway may be the pathway of IBC to induce the apoptosis of tumor cells.

Key words: isobavachalcone, tongue neoplasms, carcinoma,squamous cell, cell proliferation, apoptosis

CLC Number:

R739.86

SHI Yi, JIN Xiaohong, WU Weizhong, HUO An, ZHOU Wei, HUANG Han. Effects of isobavachalcone on proliferation and apoptosis of tongue squamous cell carcinoma Tca-8113 cells and their mechanisms[J].Journal of Jilin University Medicine Edition, 2016, 42(04): 725-730.

References

[1] Clump DA,Bauman JE,Ferris RL. Cancer of the oropharynx [J].Surg Oncol Clin N Am,2015,24(3):509-520.

[2] Chen W,Zheng R,Baade PD,et al. Cancer statistics in China,2015 [J]. CA Cancer J Clin,2016,66(2):115-132.

[3] Nishimura R,Tabata K,Arakawa M,et al. Isobavachalcone,a chalcone constituent of Angelica keiskei,induces apoptosis in neuroblastoma [J]. Biol Pharm Bull,2007,30(10):1878-1883.

[4] Jing H,Zhou X,Dong X,et al. Abrogation of Akt signaling by Isobavachalcone contributes to its anti-proliferative effects towards human cancer cells [J]. Cancer Lett,2010,294(2):167-177.

[5] Kuete V,Mbaveng AT,Zeino M,et al. Cytotoxicity of three naturally occurring flavonoid derived compounds (artocarpesin,cycloartocarpesin and isobavachalcone) towards multi-factorial drug-resistant cancer cells [J]. Phytomedicine,2015,22(12):1096-1102.

[6] Funk GF,Karnell LH,Robinson RA,et al. Presentation,treatment,and outcome of oral cavity cancer a National Cancer Data Base report [J]. Head Neck,2002,24(2):165-180.

[7] Akihisa T,Tokuda H,Hasegawa D,et al. Chalcones and other compounds from the exudates of Angelica keiskei and their cancer chemopreventive effects [J]. J Nat Prod,2006,69(1):38-42.

[8] Nowakowska Z. A review of anti-infective and anti-inflammatory chalcones [J]. Eur J Med Chem,2007,42(2):125-137.

[9] Szliszka E,Jaworska D,Ksek M,et al. Targeting death receptor TRAIL-R2 by chalcones for TRAIL-induced apoptosis in cancer cells [J]. Int J Mol Sci,2012,13(11):15343-15359.

[10] Szliszka E,Czuba ZP,Mazur B,et al. Chalcones enhance TRAIL-induced apoptosis in prostate cancer cells [J]. Int J Mol Sci,2009,11(1):1-13.

[11] Adams JM,Cory S. The Bcl-2 Protein family:arbiters of cell survival [J]. Science,1998,281(5381):1309-1312.

[12] Zhang B,Liu M,Tang HK,et al. The expression and significance of MRP1,LRP,TOPOⅡβ,and BCL2 in tongue squamous cell carcinoma [J]. J Oral Pathol Med,2012,41(2):141-148.

[13] Meterissian SH,Kontogiannea M,Po J,et al. Apoptosis induced in human colorectal carcinoma by anti-Fas antibody [J]. Ann Surg Oncol,1997,4(2):169-175.

[14] Dent P. Crosstalk between ERK,AKT and cell survival [J]. Cancer Biol Ther,2014,15(3):245-246.

[15] Briest F,Grabowski P.PI3K-AKT-mTOR signaling and beyond:the complex network in gastroenteropancreatic neuroendocrine neoplasms [J]. Theranostics,2014,4(4):336-365.

[16] Polivka J Jr,Janku F. Molecular targets for cancer therapy in the PI3K/AKT/mTOR pathway [J]. Pharmacol Ther,2014,142(2):164-175.

Effects of isobavachalcone on proliferation and apoptosis of tongue squamous cell carcinoma Tca-8113 cells and their mechanisms

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	ZHU Deqiang, CHEN Xuejun. Effects of plumbagin on proliferation and apoptosis of hepatocellular carcinoma HepG2R cells resistant to sorafenib and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1223-1229.
[2]	YAN Hui, MA Shufei, DUAN Minghua, YAN Yuli, PAN Xin, LI Haiqing, ZHOU Changlong, ZHAO Tianyi. Influence of genipin in abilities of proliferation and invasion and apoptosis of gastric cancer SGC 7901 cells and their mechanisms in vitro [J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 924-928.
[3]	XING Shugang, ZHANG Lihong, JIN Minghua, LI Baihui, WU Zexin, FANG Bo, LI Wei. Effects of myricetin on proliferation and apoptosis of glioma GL261 cells in mice [J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 955-961.
[4]	YUAN Huqin, LI Qiang. Inhibitory effects of silymarin on proliferation, migration and invasion of human gastric cancer SGC 7901 cells and their mechanisms [J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 988-993.
[5]	YU Yang, XU Lu, LIU Shibing, LI Songyan, XU Ye. Induction effect of myricetin on apoptosis of human ovarian cancer SKOV3 cells by promoting DRP1-dependent mitochondrial fission [J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 903-907.
[6]	WANG Miao, MU Runhong, LI Mingcheng, LI Hong Jie. Effects of silencing survivin and HIF-1α genes with RNAi on proliferation and apoptosis of human gastric cancer BGC-823 cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 753-758.
[7]	YANG Wanxia, SU Qiang, XING Aihua, ZHANG Xiaoyan. Effects of starvation on autophagy and apoptosis of Beclin-1-dependent Ana-1 cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 704-708.
[8]	LI Xin, MA Yunfei, TANG Geng, WEI Qi, JI Hongchi, TIAN Jiaan, SHEN Yannan, WANG Zhicheng. Enhancement of ROS induced by mitochondria-targeted KillerRed in proliferation inhibition of radiation on HeLa cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 718-723.
[9]	ZHU Xiaohui, MENG Qin, FENG Shiyan. Expression of TP53 in villus tissue of unexplained recurrent spontaneous abortion and its effect on growth of human trophoblast cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 796-800.
[10]	AN Le, YANG Jianzheng, HU Chunmei, YU Qiong, XIAO Han, HAO Shuhong, TANG Yan. Anti-apoptosis effect of polycystin-1 gene on human cervical cancer HeLa cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 776-779.
[11]	REN Yanfang, JIANG Yongjie, ZHANG Xiuling, JIANG Shan, WANG Yuhong. Effect of SHH signaling pathway on apoptosis and invasion of trophoblast cells in preeclampsia patientsand its mechanism [J]. Journal of Jilin University Medicine Edition, 2018, 44(03): 510-515.
[12]	ZHOU Liuxiang, LIN Lin, HU Xinmin, WANG Xiaochun. Promotion effect of endocrine gland-derived vascular endothelial growth factor on proliferation and migration of colon cancer LoVo cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(03): 516-520.
[13]	LYU Peng, NING Mingjie, SHAO Yu, ZHANG Luni, TANG Ying, WANG Nanbo, CHEN Feier, QI Ling. Inhibitory effect of scutellarin on proliferation of human glioma U87 cellsand its mechanism [J]. Journal of Jilin University Medicine Edition, 2018, 44(03): 466-470.
[14]	YANG Wenyan, LIU Qiang, SUN Zhijuan, DU Liqing, XU Chang, WANG Yan, LIU Yang, WANG Qin. Effect of melatonin on radiosensitivity of non-small cell lung cancer H1299 cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(03): 532-536.
[15]	SHANGGUAN Mengyuan, ZHAO Jing, YANG Yanrong, ZHANG Jiayue, CHEN Junyu, ZHAO Shuhua. Inhibitory effects of gomisin A combined with carboplatin on proliferation,invasion and metastasis of human ovarian cancer Skov3 cells and their mechanisms [J]. Journal of Jilin University Medicine Edition, 2018, 44(02): 292-298.