肝细胞生长因子和血管紧张素Ⅱ对肾小管上皮细胞转分化的影响及其作用机制

doi:10.13481/j.1671-587x.20150425

Abstract

Abstract:

Objective To explore the effects of hepatocyte growth factor (HGF) and angiotensin Ⅱ (Ang Ⅱ) on the tubular epithelial myofibroblasts (TEMT) transdifferentiation,and to clarify their mechanisms. Methods The cultured human proximal convoluted tubule HK-2 cells in vitro at stage Ⅳ were divided into control group,AngⅡ(1×10^-6mol·L^-1) group,HGF(8 μg·L^-1)group,AngⅡ(1×10^-6mol·L^-1)+HGF(8 μg·L^-1)group.The proliferation of HK-2 cells was detected by CCK8 method;the expressions of α-smooth muscle actin (α-SMA) mRNA were detected by RT-PCR and the α-SMA protein expression levels were detected by Western blotting method. Results Compared with control group,the proliferation activity of cells in AngⅡ group was decreased (P<0.01),the proliferation activity of the HK-2 cells in HGF group was increased(P<0.05),and it was also decreased in AngⅡ+HGF group (P<0.05).Compared with control group,the α-SMA mRNA and protein expression levels in the HK-2 cells in AngⅡ group were increased (P<0.05 or P<0.01), and the expression levels of α-SMA mRNA and protein in HGF group were decreased (P<0.05 or P<0.01).Compared with AngⅡ group,the proliferation activity of HK-2 cells were increased (P<0.05),and the α-SMA mRNA and protein expression levels in AngⅡ+HGF group were decreased (P<0.05). Conclusion AngⅡ can inhabit the proliferation of HK-2 cells and promote TEMT.HGF can promote the proliferation of HK-2 cells and inhabit TEMT.HGF may interfere the effects of AngⅡ on inhibiting the proliferation of HK-2 cells and promoting TEMT.

Key words: hepatocyte growth factor, tubular epithelial myofibroblasts, transdifferentiation, angiotensin Ⅱ, α-smooth muscle actin

CLC Number:

Q813

WANG Hongyue, WANG Mo, ZHANG Chen, QI Hongxin, CHI Baorong. Effects of hepatocyte growth factor and angiotensin Ⅱ on transdifferentiation of tubular epithelial-myofibroblasts and their mechanisms[J].Journal of Jilin University Medicine Edition, 2015, 41(04): 798-801.

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Effects of hepatocyte growth factor and angiotensin Ⅱ on transdifferentiation of tubular epithelial-myofibroblasts and their mechanisms

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