小窝蛋白3在人结直肠癌组织中的表达及斑蝥素对其表达的抑制作用

doi:10.13481/j.1671-587x.20150424

Abstract

Abstract:

Objective To study the caveolin-3(CAV3) protein expression in human colorectal cancer (CRC) tissue,and the effect of cantharidin on CAV3 expression,and to clarify the molecular mechanism of cantharidin in the treatment of CRC. Methods A total of 30 surgically removed CRC tissue were selected, meanwhile,the adjacent mormal tissue was collected and used as normal control group.HE staining and immunohistochemical method were used to detect the positive expression rate of CAV3 in the human CRC tissue.The human CRC HCT-116 cells were divided into control group,fluorouracil treatment group and cantharidin treatment group.The expression levels of CAV3 mRNA were detected by Real-time PCR and immunohistochemistry staining. Results The results of HE showed that the nuclei of tumor cells were stained deeply and arranged tightly.There were 22 cases among 30 cases with positive CAV3 expression,and its positive expression rate was 70.3%.The immunohistochemistry staining results showed that the number of HCT-116 cells in cantharidin group was decreased compared with control group(P<0.01);the expression levels of CAV3 in the HCT-116 cells in fluorouracil and cantharidin groups were decreased compared with control group (P<0.05).The Real-time PCR results showed that the CAV3 mRNA levels in the HCT-116 cells in fluorouracil and cantharidin groups were significantly decreased compared with control group(P<0.05). Conclusion The CAV3 protein highly expresses in the majority of human CRC tissue.Cantharidin might inhibit the growth of CRC cells through down-regulatiing the CAV3 expression.

Key words: colorectal neoplasms, caveolin-3, cantharidin

CLC Number:

R735.3

WANG Tiejun, LIU Tongjun, ZHANG Kai, SHEN Zhen, LI Kai, LI Wei, WANG Xingang, JIA Dan. Expression of caveolin-3 in human colorectal cancer tissue and inhibitory effect of cantharidin on caveolin-3 expression[J].Journal of Jilin University Medicine Edition, 2015, 41(04): 794-797.

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Expression of caveolin-3 in human colorectal cancer tissue and inhibitory effect of cantharidin on caveolin-3 expression

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