miR-122在骨髓间充质干细胞治疗大鼠急性肝损伤中的作用

doi:10.13481/j.1671-587x.20150610

Abstract

Abstract:

Objective To investigatet the effects of miR-122 in the therapy of bone marrow derived stem cells (BMSCs) for acute liver injury in the rats, and to clarify the mechanism. Methods The BMSCs were isolated from the bone marrow of male rats by density gradient centrifugation. The BMSCs were divided into transfection group and control group. The BMSCs in transfection group were transfected with miR-122 mimics by liposome, while the BMSCs in control group were not. 60 SD rats with acute liver injury induced by 10%CCl₄ were randomly divided into control group (the saline was injected through mainline), normal treatment group (the normal BMSCs were injected through mainline) and experimental therapy group (the BMSCs transfected with miR-122 mimics by liposome were injected through mainline) (n=20).The liver function and tissue pathology were examined at 1 d, 7 d and 14 d after transplantation. Results The expression level of ALB in BMSCs was up-regulated, while the AFP expression level was down-regulated after the transfection of miR-122 mimics. At 1 d after transfection of BMSCs, the serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) activities had no significant difference between normal treatment group and experimental therapy group. At 7 d and 14 d after transfection of BMSCs, the serum ALT and AST activities in experimental therapy group were obviously lower than those in normal treatment group (P<0.05). The liver congestion,cytoplasm degeneration and liver cell necrosis in experimental therapy group were improved compared with normal treatment group. Conclusion The up-regulation of miR-122 expression in BMSCs would promote its differentiation into hepatocyte like cells, which plays a role in promoting the recovery of liver injury.

Key words: bone marrow derived stem cells, miR-122, cell transplantation, liver injury

CLC Number:

R657.3

ZHAO Minxue, LI Bowen, WANG Desheng, CAO Hong. Role of miR-122 in bone marrow derived stem cell therapy for acute liver injuryin rats[J].Journal of Jilin University Medicine Edition, 2015, 41(06): 1150-1153.

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Role of miR-122 in bone marrow derived stem cell therapy for acute liver injuryin rats

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