大鼠原代背根神经元和雪旺细胞在随机及有序排列聚甲基丙烯酸甲酯电纺纳米纤维上共培养的形态学

doi:10.13481/j.1671-587x.20160101

Abstract

Abstract:

Objective: To evaluate the influence of the topographic features of polymethylmethacrylate (PMMA)electrospun nanofibers in the culture of rat dorsal root ganglion neurons (DRGn)and its coculture with Schwann cells (SCs).Methods: The random and aligned electrospun PMMA nanofibers were constructed to culture the rat DRGn or coculture it with SCs.PMMA film was used as control group. The lentivirus transfected fluorescence reporting gene was used as the coloring method,and the influence of the pographic features of PMMA electrospun nanofibers in the growth of DRGn was observed;in coculture experiment, Fast Fourier Transform of the fluorescence images and Full-width/Half maximum were calculated, and the effects of electrospun fibers on the DRGn and SCs were analyzed. Results:The DRGn and SCs were shown capable to adhere to and grow on PMMA nanofibers respectively;compared with PMMA film group, the average neurite number and average neurite lengthes of the DRGn in radnom and aligned PMMA electrospun nanofibers groups had no significant differences(P>0.05 ).In coculture experiment,PMMA electrospun nanofibers could exert obvious contact guidance on both neurite growth of DRGn and cell process growth of SCs;compared with the cells on PMMA film or random nanofibers,the FWHM of DRGn and SCs on aligned PMMA nanofibers was significantly decreased, and the aligned PMMA nanofibers could promote the neurites of DRGn and SCs to form colocalization. Conclusion: Aligned PMMA electrospun nanofibers have the potentiality as transplantable scaffold after spinal cord injury (SCI),and the topographic features may advance the process of axon myelination of SCs.

Key words: electrospun nanofibers, scaffold, dorsal root ganglion neurons, Schwann cells, contact guidance

CLC Number:

R318.08

XIA Haijian, CHEN Qiao, BO Xuenong, SUN Xiaochuan, ZHONG Dong, XIA Yongzhi. Morphology of rat dorsal root ganglion neurons and Schwann cells cocultured on polymethylmethacrylate electrospun nanofibers with different topographic features[J].Journal of Jilin University Medicine Edition, 2016, 42(01): 1-6.

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Morphology of rat dorsal root ganglion neurons and Schwann cells cocultured on polymethylmethacrylate electrospun nanofibers with different topographic features

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