Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (03): 617-621.doi: 10.13481/j.1671-587x.20160339

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Preparation of total flavonoids of chrysanthemum indicum(TFC)-PLGA sustained release microspheres and its optimization of technology

LU Qing, CHE Hongze, CHEN Nannan, CHEN Fei, JIAO Peng, MA Ning   

  1. Department of Periodontology, Stomatology Hospital, Jilin University, Changchun 130021, China
  • Received:2016-03-09 Published:2016-06-17

Abstract:

Objective: To prepare the sustained release microspheres of total flavonoids of chrysanthemum indicum(TFC)-polyactic-co-glycolic acid(PLGA) and to optimize the conditions. Methods: The TFC-PLGA microspheres were prepared by using the double emulsion solvent evaporation method.The effects of different conditions,such as the stirring rate,the polyvinyl alcohol(PVA) concentration and the ratio of TFC to PLGA on the entrapment efficiency(EE) of microspheres were observed.The general morphology of the microspheres was observed by microscope;the surface morphology and particle size of the microspheres were observed by SEM;the EE and in vitro release results of the microspheres were measured by UV spectrophotometry. Results: When the stirring rate was 3 000 r·min-1,the PVA concentration was 3.0%,and the ratio of of TFC to PLGA was 1:15 as the optimal conditions,the acquired TFC-PLGA microspheres showed well-defined properties,with uniform sphere in appearance,general particles without adhesion,and the average EE was (45.03±1.25)% and the mean diameter was (102.20±1.97)μm.The results of release experiment in vitro showed that the percentage of accumulative release were 22.07% in 24 h and 92.32% on 20 d and the micropheres had significant sustained release effect. Conclusion: The TFC-PLGA microspheres with suitable size, uniform dispersion and high EE can be acquired under the optimized conditions.

Key words: total flavonoids of chrysanthemum indicum, polyactic-co-glycolic acid, microspheres, sustained release

CLC Number: 

  • R285