Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (04): 690-693.doi: 10.13481/j.1671-587x.20160411

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Application of 9.4 T 1H-MR spectroscopy in determination of lactate in brain of mice with early acute hypoxia-ischeia injury and its significance

ZHANG Yunping1, LI Yangkang2, LIU Chenyang3, ZHU Jiahui3, WU Renhua4   

  1. 1. Department of Nuclear Medicine, Affiliated Tumor Hospital, College of Medical Sciences, Shantou University, Shantou 515031, China;
    2. Department of Radiology, Affiliated Tumor Hospital, College of Medical Sciences, Shantou University Shantou 515031, China;
    3. Department of Nuclear Medicine, Taixing People's Hospital, Jiangsu Province, Taixing 225400, China;
    4. Teaching and Research Section of Medical Imaging, College of Medical Sciences Shantou University, Shantou 515031, China
  • Received:2015-11-14 Published:2016-07-20

Abstract:

Objective: To explore the lactate metabolism in brain tissue of the mice with early acute hypoxia-ischemia injury,and to provide data support for 9.4T 1H-NMR spectroscopy in detecting the lactate level clinically. Methods: Eighty Kunming mice were randomly divided into sixteen groups (0 s,20 s,40 s,60 s,2 min,4 min,6 min,8 min,10 min,12 min,14 min,16 min,18 min, and 20 min) according to the duration of hypoxia-ischemia(n=5).The changes of lactate levels were detected by 9.4T 1H-NMR spectroscopy. Results: After the initiation of hypoxia-ischemia injury,the lactate level began to increase rapidly to the highest value of (6.89±0.34) μmol·g-1 at 20 s,then started to decline quickly from 40 s to 2 min,and eventually decreased to a stable level of (4.85±0.36) μmol·g-1 until 6 min.Compared with control group,the levels of lactate in brain tissue of the mice in hypoxic-ischemic groups were increased(P < 0.01). Conclusion: 40 s of acute hypoxia-ischemia may be the lactate cerebral neuron threshold during the anaerobic glycolysis. 9.4T1H-MRS can provide the exact time window for detecting the lactate metabolism.

Key words: acute cerebral ischemia, lactate, cerebral metabolites, proton magnetic resonance spectroscopy

CLC Number: 

  • R445.2