Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (04): 699-703.doi: 10.13481/j.1671-587x.20160413

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Effect of activin A on regulation of arterial blood pressure by hypothalamic paraventricular nucleus and its mechanism

FAN Yuqi1, WANG Minghua1, XIE Dongxue2, CUI Xueling2, CUI Xueling1   

  1. 1. Department of Physiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China;
    2. Department of Genetics, School of Basic Medical Sciences, Jilin University, Changchun 130021, China
  • Received:2016-01-25 Published:2016-07-20

Abstract:

Objective: To investigate the expression of activin A in paraventricular nucleus (PVN) of the WKY rats and its influence in arterial blood pressure,and to clarify the mechanism of activin A in the regulation of arterial blood pressure by PVN. Methods: The WKY rats were selected. The expressions of activin A,ActRⅡA,ActRⅡB,and Smads mRNA in PVN of the WKY rats were measured by RT-PCR. The expression of ActRⅡA protein in PVN was detected by immunohistochemical staining. The microinjection of exogenous activin A into PVN was used to observe the changes of arterial blood pressure. The primary cultured PVN neurons from the WKY rats were divided into control group and activin A group. The mRNA expression levels of ActRⅡA,ActRⅡB, and Smads in the PVN neurons were analyzed by RT-PCR. Results: Activin A,ActRⅡA,ActRⅡB,Smad2 and Smad3 mRNA were expressed in PVN of the WKY rats. The ActRⅡA protein expression in PVN was further confirmed by immunohistochemical staining. After microinjection of activin A or angiotensin Ⅱ (AgⅡ) into PVN,the mean arterial blood pressure was increased obviously compared with before treatment (P < 0.05). Moreover,compared with control group,the expression levels of ActRⅡA and Smad3 mRNA in primary cultured PVN neurons of the rats in vitro were significantly increased(P < 0.05). Conclusion: Activin A can regulate the arterial blood pressure in PVN in an autocrine or paracrine manner,which is related to ActRⅡA-Smad3 signal pathway.

Key words: arterial blood pressure, paraventricular nucleus, activin A, drosophia mothers against decapentaplegic protein

CLC Number: 

  • R-331