富血小板血浆对创伤性颅脑损伤大鼠神经功能的保护作用

doi:10.13481/j.1671-587x.20160514

Abstract

Abstract:

Objective: To study the protective effect of platelet-rich plasma on never function in the rats with traumatic brain injury(TBI),and to clarify its mechanisms. Methods: Sixty SD rats were randomly divided into sham group(opened skull bone window only), TBI group and platelet-rich plasma treatment group(PRP group)(n=20). The rats in PRP group were injected with platelet-rich plasma through vessel on the 1st day, the 2nd day and the 6th day after operation while the rats in sham group and TBI group were treated with saline at the same time. The neurological function defects were assessed with modified neurological severity score(mNSS) on the 1st, 3rd and 7th after operation. Then 10 rats were taken from each group and executed, and the brain tissues were taken. The brain sections were prepared for the histological observation and the others of each group were tested with Morris water maze. Results: Compared with sham group,the mNSS scores of the rats in TBI and PRP group were increased (P<0.05); the mNSS score of the rats in PRP group was decreased compared with TBI group(P<0.05). The injured volume of rat brain tissue was reduced significantly in PRP treated group compared with TBI group (P<0.05). The Nissl staining results showed that the injury area in PRP group had a more neat rows and a larger number of new blood vessels compared with TBI group. The immunohistochemical staining results showed the injured area had a higher level expression of GFAP⁺ cells in TBI group compared with PRP group, but the amount of neuN⁺ cells was smaller than that in PRP group(P<0.05). The Morris water maze test results showed that there were a shorter escape latency time, more times acrossing platform and a larger swimming time during platform quadrant in PRP group compared with TBI group(P<0.05). Conclusion: Platelet-rich plasma has a significant role in protecting the neurological function of TBI rats.

Key words: traumatic brain injury, platelet-rich plasma, neurological function

CLC Number:

R651.15

WANG Yadong, LI Dongpeng, GUO Dewei, SONG Jishi, LI Hongwei, QIAN Weiqiang, YANG Bo. Protective effect of platelet-rich plasma on never function in rats with traumatic brain injury[J].Journal of Jilin University Medicine Edition, 2016, 42(05): 910-914.

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Protective effect of platelet-rich plasma on never function in rats with traumatic brain injury

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