SOX2对牙髓干细胞神经分化的促进作用及其意义

doi:10.13481/j.1671-587x.20160607

Abstract

Abstract:

Objective: To explore the effect of SOX2 on the neural differentiation of dental pulp stem cells (DPSCs), and to provide basis for studying the mechanism of neural differentiation of DPSCs.Methods: The DPSCs (DPSCs group) were isolated from human dental pulp of third molar, and the SOX2 over exression DPSCs (DPSCs-SOX2 group) were established via retrovirus infection. The empty vector-infected DPSCs (DPSCs-vector group) were regarded as control group. The DPSCs in various groups were induced into neural progenitor cells (NPCs) by neural differentiation medium. The proliferation index (PI) of NPCs in various groups was evaluated by CCK-8 method; the expression levels of Nestin and Pax6 gene in NPCs in various groups were detected by qPCR and FACS; the differentiation abilities of NPCs and the expression efficiencies of β3-Tubulin after differentiation in various groups were evaluated by immunofluorescence and FACS methods.Results: All of normal DPSCs, DPSCs-vector and DPSCs-SOX2 derived from NPCs could be differentiated into NPCs successfully through the induction of neural differentiation medium. Compared with DPSCs and DPSCs-vector groups, the PI of DPSCs in DPSCs-SOX2 group was increased (P<0.05), and the expression levels of Nestin and Pax6 in NPCs were increased (P<0.05). The expression efficiency of β3-Tubulin in differentiated NPCs in DPSCs-SOX2 group was higher than those in DPSCs and DPSCs-vector groups (P<0.05).Conclusion: SOX2 could have a certain promotive effect on the neural differentiation of DPSCs.

Key words: dental pulp stem cells, cell therapy, sex determining region Y-box 2, neural differentiation, neural progenitor cells, cell proliferation

CLC Number:

R329.2

YUAN Jing, CHEN Yaoyu, ZHAO Yifan, LIU Xiaobo, LIU Pengfei, ZHAO Lei. Promotive effect of SOX2 on neural differentiation of dental pulp stem cells and its significance[J].Journal of Jilin University Medicine Edition, 2016, 42(06): 1076-1080.

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Promotive effect of SOX2 on neural differentiation of dental pulp stem cells and its significance

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