Abstract:

Objective: To establish and evaluate the model of liver regeneration of the cirrhosis mice after partial hepatectomy.Methods: Fifty mice were randomly divided into experiment group(n=25) and control group(n=25). The mice in experiment group were injected with CCl₄ intraperitoneally twice per weekfor 8 weeks and the mice in control group were injected with soya oil.Partial hepatectomy of 37% liver was performed on all mice in two groups. The successful rate of operation, survival rate after operation, survival statue after operation and postoperative liver regeneration rate of the mice in two groups were observed; the pathologic characteristics of liver tissue of the mice in two groups were observed by HE staining and Masson staining;the levels of serum hepatocyte growth factor (HCG) and alanine aminotransferase (ALT) of the mice in two groups were detected by ELASA methods.Results: The successful rates of operation of the mice in two groups were both 100%, and the survival rates of the mice at 6 weeks after operation were 100%.During 3 weeks after operation, the regeneration rates of liver tissue of the mice in experiment group were lower than those in control group; the regeneration rate of liver tissue of the mice in experiment group was equal to that in control group at 3 weeks after operation;the regeneration rates of liver tissue of the mice in experiment group were higher than those in control group from 3 weeks after operation. The HE staining and Masson staining results showed that the liver tissue presented the pathological changes of cirrhosis 8 weeks after injection with CCl₄;after partial hepatectomy, the structures of liver tissue were gradually improved. The levels of serum HGF of the mice in experiment group were significantly higher than those in control group; the levels of serum HGF of the mice in two groups were increased compared with the preoperative levels, and then recovered to the preoperative level. Compared with control group, the levels of serum ALT of the mice in experiment group after hepatectomy were increased significantly, and the duration was longer and the time to reach the peak was delayed.Conclusion: It is extremely easy to establish a stable and reliable liver regeneration model of liver cirrhosis mice after partial hepatectomy by the resection of left lateral lobe of the cirrhosis mice induced by CCl₄ with silk ligation.

Key words: carbon tetrachloride, cirrhotic liver, disease model,animal, mice,ICR, hepatectomy