脉管Ⅱ号胶囊对大鼠后肢缺血后血管再生的影响及其机制

doi:10.13481/j.1671-587X.20210216

Abstract

Abstract: Objective

To explore the effect of Vascular Capsule Ⅱ on the limb ischemia of the rats， and to lay a theoretical foundation for the treatment of peripheral arteriopathy.

Methods

A total of 60 SPF female SD rats were randomly divided into normal control group， model group， sham operation group， positive control group （0.202 5 g·kg^－1 Compound Danshen Tablets）， low dose of Vascular Capsule Ⅱ group （30 mg·kg^－1） and high dose of Vascular Capsule Ⅱ group （90 mg·kg^－1）；there were 10 rats in each group. The models of hind limb ischemia were established by ligating the proximal， distal and main branches of femoral artery and vein. The general states of rats were observed. The pathomorphology of hind limb muscle tissue of the rats in various groups was observed by HE staining. The number of cells with positive expression of angiopoietin 1 （Ang-1）， tyrosine kinase receptor 2 （Tie2）， and CD31 in muscle tissue of the rats in various groups were detected by immunohistochemistry. The expression levels of Ang-1 and Tie2 proteins in muscle tissue of the rats in various groups were detected by Western blotting method.

Results

The skin temperature of the hind limbs of the operation rats was low， the skin color was dark red， and cyanosis and toenail dropping occurred in the hind limbs. Compared with normal group and sham operation group， the hind limb muscle cells of the rats in model group were obviously swollen， the transverse striae disappeared， and the myofibril atrophy in the perineurium was observed；compared with model group，the symptoms mentioned above in positive control group and low and high doses of Vascular Capsule Ⅱ groups were alleviated.Compared with sham operation group，the number of cells with positive expressions of Ang-1 and Tie2 in muscle tissue of the rats in model group was significantly decreased（P<0.05）；compared with model group，the number of cells with positive expressions of Ang-1 and Tie2 in muscle tissue of the rats in positive control group and low and high doses of Vascular Capsule Ⅱgroups were significantly increases（P<0.05）. Compared with model group， the skin temperature differences of hind limbs of the rats in positive control and high dose of Vascular Capsule Ⅱ group were significantly decreased （P<0.05）， and the expression levels of CD31， Ang-1， and Tie2 proteins were significantly increased （P<0.05）.

Conclusion

Vascular Capsule Ⅱ can promote the vascular regeneration after limb ischemia in the rats，and its mechanism may be related to activating the Ang-1/Tie2 pathway.

Key words: Vascular Capsule Ⅱ, limb ischemia, angiopoietin-1, tyrosine kinase receptors 2, vascular regeneration

CLC Number:

R285.5

Shijun WANG,Weilian YANG,Fuwei CHEN,Meiji ZHANG,Xu YANG. Effect of Vascular Capsule Ⅱ on vascular regeneration after hind limb ischemia in rats and its mechanism[J].Journal of Jilin University(Medicine Edition), 2021, 47(2): 369-376.

Figures/Tables 7

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References 24

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Group	Number of positive cells
Group	Ang-1	Tie2
Normal control	0.36±0.04	0.33±0.01
Sham operation	0.36±0.04	0.31±0.01
Model	0.25±0.02^*	0.23±0.02^*
Positive control	0.32±0.02^△	0.30±0.03^△
Low dose of Vascular CaspuleⅡ	0.25±0.04	0.28±0.02
High dose of Vascular CaspuleⅡ	0.30±0.02^△	0.29±0.02^△

Group	A value
Group	Ang-1	Tie2
Normal control	1.00±0.10	1.00±0.05
Sham operation	0.96±0.14	0.94±0.05
Model	0.39±0.11^*	0.38±0.05^*
Positive control	0.75±0.09^△	0.78±0.06^△
Low dose of Vascular CaspuleⅡ	0.51±0.06^△	0.53±0.09^△
High dose of Vascular CaspuleⅡ	0.72±0.09^△	0.73±0.08^△

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Effect of Vascular Capsule Ⅱ on vascular regeneration after hind limb ischemia in rats and its mechanism

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