Journal of Jilin University(Medicine Edition) ›› 2023, Vol. 49 ›› Issue (2): 298-307.doi: 10.13481/j.1671-587X.20230205
• Research in basic medicine • Previous Articles Next Articles
Yunpeng LIU1,Zihao LIU1,Boming KANG2,Zhiguang YANG1()
Received:
2022-06-27
Online:
2023-03-28
Published:
2023-04-24
Contact:
Zhiguang YANG
E-mail:zgyang@jlu.edu.cn
CLC Number:
Yunpeng LIU,Zihao LIU,Boming KANG,Zhiguang YANG. Regulatory effect of lncRNA SNHG17 on biological behavior of non-small cell lung cancer cells by targeting AEG1 through miR-384[J].Journal of Jilin University(Medicine Edition), 2023, 49(2): 298-307.
Tab. 1
Expression levels of lncRNA SNHG17, cell viabilities and numbers of migration and invasion cells in various groups after silencing lncRNA SNHG17 or over-expression of lncRNA SNHG17"
Group | lncRNA SNHG17 | Cell viability (η/%) | Number of migration cells | Number of invasion cells |
---|---|---|---|---|
si-NC (A549 cells) | 1.03±0.24 | 95.32±5.45 | 384.00±12.00 | 191.00±5.03 |
si-SNHG17 (A549 cells) | 0.21±0.02* | 45.68±10.22* | 289.00±6.08* | 111.00±3.06* |
pcDNA3.1-NC (H1299 cells) | 1.02±0.19 | 96.56±3.89 | 499.00±13.53 | 147.00±6.11 |
pcDNA3.1-SNHG17 (H1299 cells) | 6.35±1.42△ | 136.23±10.93△ | 716.00±11.27△ | 271.00±7.21△ |
Tab. 2
Expression levels of miR-384 and AEG1 mRNA in cells in various groups after silencing lncRNA SNHG17 or over-expression of lncRNA SNHG17"
Group | miR-384 | AEG1 mRNA |
---|---|---|
si-NC(A549 cells) | 1.01±0.10 | 1.03±0.24 |
si-SNHG17(A549 cells) | 1.46±0.20* | 0.06±0.02* |
pcDNA3.1-NC(H1299 cells) | 1.01±0.13 | 1.02±0.19 |
pcDNA3.1-SNHG17(H1299 cells) | 0.64±0.14△ | 2.34±0.27△ |
Tab. 3
Expression levels of miR-384, cell viabilities, numbers of migration and invasion cells in various groups"
Group | miR-384 | Cell viability (η/%) | Number of migration cells | Number of invasion cells |
---|---|---|---|---|
Inhibitor NC(A549 cells) | 1.02±0.21 | 97.16±4.22 | 417.00±11.14 | 160.00±7.00 |
miR-384 inhibitor(A549 cells) | 0.02±0.01* | 145.69±22.24* | 489.00±16.64* | 243.00±3.79* |
Mimics NC(H1299 cells) | 1.02±0.21 | 96.37±3.22 | 518.00±11.53 | 207.00±6.03 |
miR-384 mimics(H1299 cells) | 4.68±1.00△ | 65.67±6.40△ | 406.00±17.67△ | 116.00±8.00△ |
Tab. 4
Cell viabilities and numbers of migration and invasion cells in various groups after inhibiting or enhancing lncRNA SNHG17 and miR-384"
Group | Cell viability (η/%) | Number of migration cells | Number ofinvasion cells | Expression level of AEG1 protein |
---|---|---|---|---|
si-NC+inhibitor NC(A549 cells) | 98.07±4.84 | 364.00±18.15 | 195.00±9.85 | 1.00±0.03 |
si-SNHG17+inhibitor NC(A549 cells) | 63.26±6.04* | 314.00±8.72* | 108.00±6.11* | 0.72±0.01* |
si-SNHG17+miR-384 inhibitor(A549 cells) | 78.69±6.65△ | 350.00±8.74△ | 199.00±11.15△ | 0.81±0.01△ |
pcDNA3.1-NC+mimics NC(H1299 cells) | 101.26±5.91 | 496.00±18.23 | 148.00±4.58 | 1.00±0.02 |
pcDNA3.1-SNHG17+mimics NC(H1299 cells) | 138.61±9.05# | 674.00±6.93# | 271.00±9.07# | 2.26±0.04# |
pcDNA3.1-SNHG17+miR-384 mimics(H1299 cells) | 108.64±7.87○ | 582.00±14.53○ | 221.00±3.79○ | 1.56±0.02○ |
Tab. 5
Expression levels of AEG1 mRNA in cells, cell viabilities and numbers of migration and invasion cells in various groups after inhibiting or enhancing AEG1"
Group | AEG1 mRNA | Cell viability (η/%) | Number of migration cells | Number of invasion cells |
---|---|---|---|---|
si-NC(A549 cells) | 1.02±0.22 | 98.67±4.76 | 381.00±13.08 | 167.00±6.03 |
si-AEG1(A549 cells) | 0.56±0.08* | 71.32±8.98* | 308.00±10.26* | 115.00±10.02* |
pcDNA3.1-NC(H1299 cells) | 1.01±0.11 | 99.21±7.66 | 499.00±11.53 | 137.00±7.09 |
pcDNA3.1-AEG1(H1299 cells) | 3.15±0.90△ | 132.32±9.33△ | 609.00±11.14△ | 282.00±3.79△ |
Tab. 6
Cell viabilities and numbers of migration and invasion cells in various groups after inhibiting or enhancing miR-384 and AEG1 mRNA"
Group | Cell viability (η/%) | Number of migration cells | Number of invasion cells |
---|---|---|---|
Inhibitor NC+si-NC(A549 cells) | 101.36±7.40 | 408.00±12.58 | 161.00±6.11 |
miR-384 inhibitor+si-NC(A549 cells) | 145.37±19.97* | 481.00±8.66* | 256.00±9.17* |
miR-384 inhibitor+si-AEG1(A549 cells) | 112.36±7.47△ | 425.00±9.07△ | 172.00±9.07△ |
Mimics NC+pcDNA3.1-NC(H1299 cells) | 98.63±8.83 | 520.00±8.14 | 162.00±6.03 |
miR-384 mimics+pcDNA3.1-NC(H1299 cells) | 63.17±9.12# | 401.00±10.44# | 117.00±2.08# |
miR-384 mimics+pcDNA3.1-AEG1(H1299 cells) | 81.06±5.75○ | 589.00±6.24○ | 142.00±8.71○ |
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