Abstract: Objective: To investigate the effect of endocrine gland-derived vascular endothelial growth factor(EG-VEGF) on the malignant behavior of colon cancer LoVo cells. Methods: The LoVo cells treated with different concentrations (50,100,and 200 μg·L^-1)of EG-VEGF were used as EG-VEGF groups,and the colon cancer LoVo cells cultured with solution without EG-VEGF were regarded as control group. The proliferation activity of LoVo cells was determined by MTT method; flow cytometry(FCM) was used to detect the percentages of LoVo cells at different cell cycles in various groups; Wound scratch assay test and Transwell cell migration assay were used to detect the migration rates of LoVo cells and the number of migration cells. Results: The MTT results showed that compared with control group,the proliferation activities of LoVo cells in 50,100,and 200 μg·L^-1EG-VEGF groups were increased significantly (P<0.05); with the increasing of concentration of EG-VEGF,the proliferation activity was increased apparently. The FCM results showed that compared with control group,the percentage of colon cancer LoVo cells at G₀/G₁ phase in 100 μg·L^-1EG-VEGF group was decreased (P<0.05) and the percentage of colon cancer LoVo cells at S phase was increased (P<0.05),but the percentage of colon cancer LoVo cells at G₂+M phase did not change significantly.The wound scratch assay results showed that compared with control group,the migration rate of LoVo cells in 100 μg·L^-1EG-VEGF group was increased (P<0.05). The Transwell assay results showed that compared with control group,the number of migration cells in 100 μg·L^-1 EG-VEGF group was increased significantly (P<0.05). Conclusion: EG-VEGF can obviously promote the proliferation and migration of colon cancer LoVo cells,and EG-VEGF may be associated with the malignant development of colon cancer.

Key words: migration, endocrine gland-derived vascular endothelial growth factor, cell cycle, cell proliferation, colon neoplasms