Abstract: Objective: To investigate the anti-fatigue phenomenon induced by forced swimming in the mice,and to explore the anti-fatigue effect of argininyl fructoyl glucose(AFG)from red ginseng in the mice and its mechanism. Methods: The AFG was extracted from red ginseng. The ICR mice were divided into blank control group,low dose of AFG group(100 mg·kg^-1),middle dose of AFG group(200 mg·kg^-1) and high dose of AFG group(400 mg·kg^-1)(n=20). The mice mere given a forced swimming test after continuous gavage for 28 d. The weights,organ indexes,time of forced swimming,contents of lactic acid (LD),blood urea nitrogen (BUN),hepatic glycogen (Gly) and expressing levels of PGC-1α in gastrocnemius of the mice in various groups were detected. Results: Compared with blank control group,the weights and organ indexes of the mice in low,middle and high doses of AFG groups had no significant differences(P>0.05). Compared with blank control group,the time of forced swimming,contents of Gly and expressing levels of PGC-1α of the mice in low,middle and high doses of AFG groups were significantly increased(P<0.05 or P<0.01)in a dose-dependent manner. Compared with blank control group,The contents of LD and BUN in serum of the mice in low,middle and high doses of AFG groups were significantly decreased(P<0.01). Conclusion: AFG has anti-fatigue effect in mice,and its mechanism may be related to energy metabolism.

Key words: red ginseng, exhaustive swimming training, real-time fluorescence quantitative polymerase chain reaction, Western blotting method, arginyl fructosyl glucose