Abstract:

To study the expression levels of the Toll like receptor(TLRs) regulated by DNA-PKcs and autoimmune regulator in peritoneal macrophages in mice,and to clarify the function and significance of autoimmune regulator in peripheral immune system. Methods The macrophages were divided into pEGFPC1/mAire transfection group,pEGFPC1/mAire and negative control siRNA co-transfection group,pEGFPC1/mAire and DNA-PKcs siRNA co-transfection group,pEGFPC1 and negative control siRNA co-transfection group,and  pEGFPC1 and DNA-PKcs siRNA co-transfection group;the expression levels of TLR1-9 in peritoneal macrophages of the mice in various groups  were detected by RT-PCR. The expression levels  of TLR1-9 in peritoneal macrophages were detected by RT-qPCR after transfected with pEGFPC1/mAire and pEGFPC1 into the peritoneal macrophages in mice;the expression levels of TLRs in the transfected cells  before and after silencing   DNA-PKcs  were detected by RT-qPCR. Results The peritoneal macrophages in mice could express TLR1-9. Compared with  pEGFPC1/mAire transfection group,the expression levels of TLR1,3 and 8 were increased in peritoneal macrophages after transfected with autoimmune regulator(P<0.05) and those in the other groups did not changed significantly(P>0.05). After silencing  DNA-PKcs,the expression levels of TLR1,3,and 8 were decreased (P<0.05) in peritoneal macrophages in pEGFPC1/mAire transfection group than thoes in unsilenced group,but the expression levels of TLR1-9 didn’t  have significant changes in macrophages transfected with pEGFPC1 before and after  silence(P>0.05). Conclusion  Autoimmune regulator can regulate the expression levels of TLR1,3,and 8 in peritoneal macrophages in mice,and the mechnism may be  associated with  DNA-PKcs interaction effect.

Key words:  autoimmune regulator；macrophages；Toll-like receptor；partner