Abstract:

To observe the effects of Mongolian Remedy RuXian- I(M-Ⅰ) on anti- oxygenic ability and the expressions of estrogen receptor(ER) and progesterone receptor(PR)  in breast tissues of rats with hyperplasia of mammary glands,and to provide experimental basis for application of M-Ⅰ.Methods 8 rats randomly chosen from forty-eight virgin female Wistar rats were regarded as normal control group.The others were injected intramuscularly with estradiol benzoate (0.5 mg·kg^-1) once a  day for 25 d and progesterone(4 mg·kg^-1) once a  day for 5 d.The rats in normal control group were injected with normal saline.Then the disease model  was sucesfully copied and the model rats were randomly divided into model control group,positive control group,M-Ⅰ low dosage group,M-Ⅰ middle dosage group,and  M-Ⅰ high dosage group.The rats in different groups were respectively treated with normal saline,tamoxifen,M-Ⅰ 0.5,1.0 and 3.0 g·kg^-1for 30 d.Then the changes of nipple heights,the pathological changes in breast tissues,the levels of SOD,MDA,GSH-Px in serum and breast tissues and the expressions of ER,PR were measured. Results Compared with normal control group,the nipple height and the levels of serum MDA,and the expressions of  ER and PR in model control group were obviously increased(P<0.05 or P<0.01);the serum levels of SOD,GSH-Px were obviously decreased（P<0.01).Compared with model control group,the   nipple heights were lower(P<0.05 or P<0.01),and the  histopathological changes were reduced in  M-Ⅰ different dosages groups;the structure of breast tissue in M-Ⅰ high dosage group was close to that in normal control group;the serum levels of MDA and the expressions of ER and PR in breast tissue in M-Ⅰ high dosage group were obviously decreased(P<0.05 or P<0.01),but the levels of SOD,GSH-Px in serum and breast tissues were obviously increased(P<0.05 or P<0.01).Conclusion  M-Ⅰ can treat the rats with hyperplasia of mammary glands well,and its mechanism may be related to inhancing  the antioxidant ability and  inhibiting the  expression  levels of ER and PR in  breast tissues.

Key words: rat,Wistar；Mongolian Remedy RuXian-Ⅰ；estrogen；hyperplasia of mammary glands； estrogen receptor