Abstract:

Abstract:Objective To discuss the relationship between the expression of death-inducing signaling complex (DISC) and apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand (TRAIL) in malignant glioma cells,and to explore the mechanism of resistance of TRAIL initiated apoptosis. Methods The primary cultured cells were isolated from human malignant glioma tissues,and  the level of apoptotsis was detected by acid phosphatase assay after the  cells  treated with different doses of TRAIL; and  the level of DISC protein was determined by Western blotting.Results The primary malignant glioma cells GC417,GC321 and GC125 were isolated and cultivated,and the sensitivities of the cells to TRAIL were different,GC321 (0.12±0.01  vs  0.51±0.02) and GC125 (0.22±0.01  vs  0.36±0.01) were sensitive to TRAIL,there were significant differences compared with control group(P<0.01);but GC417 (0.24±0.01  vs  0.23±0.02 ) was resistant to TRAIL.The results of Western blotting showed that the expressions of DISC proteins were various,and the expressions of GC321 and GC125 were increased,and the expression of GC417 was decreased.Conclusion The responses are different according to the different primary malignant glioma cells to TRAIL-induced apoptosis,and the expressions of DISC proteins are either different; there is relationship between the decreasing of the  expressions of DISC proteins and the  resistance of TRAIL initiated apoptosis.

Key words: glioma, death-inducing signaling complex, tumor necrosis factor related apoptosis-inducing ligand, apoptosis