Abstract:

Abstract:Objective To optimize the prescription and preparation of recombinant human acidic fibroblast growth factor(rhaFGF) liposomes,and to study the physical and chemical properties. Methods The encapsulation efficiency and biological activity of rhaFGF liposomes were evaluated by single factor test. Freeze-drying-rehydration was used to obtain the optimum prescription and preparation of rhaFGF liposomes. Results The encapsulation efficiency of rhaFGF liposomes was (81.79±2.51)%,the physical stability index (KE) was (1.050±0.342)% and the bioactivity was (6.521 0±0.617 1)×105U/mL. The particle size was about 100 nm uniformly. Conclusion The preparation of rhaFGF liposomes is optimized,and these liposomes have high encapsulation efficiency,good physical stability and high bioactivity at 4℃.

Key words:  recombinant human acidic fibroblast growth factor, liposome, encapsulation efficiency, percolation ratio