Abstract:

Abstract:Objective To study the inhibitory effect of mistletoe alkali on the growth and invasiveness of human osteosarcoma cells (U2OS) in vitro. Methods The effects of mistletoe alkali on the growth of U2OS cells were detected by CCK-8 kit. The U2OS cells treated by 5-FU were used as positive control group and the U2OS cells without any treatment as  negative control group, the U2OS cells treated by mistletoe alkali were used as experimental groups. Meanwhile the growth of U2OS cells after treated by mistletoe alkali was assessed by cell growth curve. And the morphological changes of U2OS cells under different concentrations of medicine were observed. Furthermore, Their invasion ability was tested with Boyden chamber. Results Mistletoe alkali inhibited the growth of U2OS cells and the value of IC50 was 7 mg·L^-1. After treatment, the growth of U2OS cells slowed, and the cells became round, adherence decreased. Furthermore, the invasion ability of cells in the experiment group was obviously decreased. Conclusion Mistletoe alkali obviously inhibits the growth and the invasion ability of U2OS cells in vitro.

Key words: mistletoe alkali；osteosarcoma；inhibit；growth；neoplasm invasiveness