Abstract: To study the effects of broccoli polypeptide on the proliferation of C6 glioma cells of rats,and to discuss the induction of broccoli polypeptide on apoptosis of tumor cells.Methods The crude broccoli peptide was ectracted,separated and purificated.The rat C6 glioma cells were cultivated and  treated with different doses of broccoli polypeptide (0,0.01,0.10,1.00,10.00 mg•L^-1) for 24,48 and 72 h,and the apoptosis of cells was observed under inverted microscope,then the growth of C6 glioma cells after treated with broccoli polypeptide  was detected by MTT assay.Results  Broccoli polypeptide had  four main eluting peaks,and the second one (partⅡ) was rich in polypeptide which was used for continued study.The apoptotic bodies were found  under inverted microscope after the C6 glioma cells were treated with broccolipolypeptide,especially in 10.00 mg•L^-1 group.The MTT results showed that after treated  for 48 h,the proliferation ability of cells  in 10 mg•L^-1 group (0.127±0.011) was significantly decreased compared with control group(0.501±0.035,P<0.01).After treated for 72 h,all doses of polypeptide inhibited the growth of cells;compared with control group(0.753±0.022),the proliferation abilities of the cells in 1.00 mg•L^-1 group (0.583±0.082) and 10.00 mg•L^-1  group (0.073±0.001) were significantly decreased(P<0.01).Conclusion Broccoli polypeptide may induce the apoptosis of C6 glioma cells,and the effect is increased with the prolengation of the treatment time and the increasing of drug dose,which suggesting
 that broccoli polypeptide can  induce the apoptosis of tumor cells.

Key words: broccoli polypeptide, glioma cells, apoptosis