Journal of Jilin University Medicine Edition

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Expressions of HIF-1α  and RelA in mononuclear cells in peripheral 
blood of health adults after ascentting of plateau and their 
correlations with pathogenesis of acute mountain sickness

FANG Pan-pan1,2,GONG Cui-cui1,2,CANG Bao-cheng1,2,LI
Zheng-min1,2,LI Zong-jie1,2,LI Wen-li2,ZHU Chuan-jie2,WANG Ying-tao2   

  1. 1.Scientific Research  Center,Third Affiliated Hospital,Zhengzhou Unive
    rsity,Zhengzhou 450052,China; 2. Laboratory Center,No. 153 Hospital of PLA,Zhengzhou 450042,China
  • Received:2013-01-30 Online:2013-07-28 Published:2013-08-17

Abstract:

Abstract:Objective To observe the  changes of expression levels of  hypoxia-inducible factor 1 alpha(HIF-1α)and nuclear factor kappa B(NF-κB) p65 (RelA) in
mononuclear cells  after ascenting of plateau  and their correlations with the incidence
 rate of acute mountain sickness(AMS),and to explore the role and mechanism of
 HIF-1α  and RelA in pathogenesis of AMS.Methods The soldiers’ venous blood before ascentting of plateau and 48 h after arriving Yushu were collected,and the p
rotein and mRNA in mononuclear cells were extracted.The expressions of HIF-1α and RelA mRNA were determined by RT-PCR.The expression of HIF-1α  protein was
determined by Western blotting method.The correlations between the expressions of HIF-1α  and RelA and pathogenesis of  AMS were analyzed with Spearman anal
ysis.Results Compared with before ascentting,after ascentting of plateau the expression levels of  HIF-1α mRNA,RelA mRNA and
 HIF-1α   protein  were increased significantly(P<0.01).The expression of HIF-1α mRNA was positively correlated with the  RelA mRNA expression
(r=0.806,P<0.01).At the same time,the expression  of HIF-1α  protein was positively  correlated with the pathogenesis of AMS(r= 0.875,P<0.01);and the expression levels of HIF-1α  and RelA had no significant difference between women and men(P>0.05).Conclusion
The expression level of HIF-1α  protein is increased significantly after asentting of plateau,and has significantly positive correlation with the pathogenesis of AMS.The expression levels of HIF-1α  and RelA mRNA are significantly increased 48 h after ascentting of plateau,and they have positive correla
tion,prompting that the existence of cross-talk phenomenon between their signal transduction pathways.

Key words: acute mountain sickness, hypoxia-inducible factor 1 alpha , nuclear factor kappa B p65, hypoxia, cross-talk

CLC Number: 

  • R394.3