Abstract:

Abstract:Objective To study the effects of formaldehyde on apoptosis and cell cycle of testicle cells in male mice,and explore the mechanism of formaldehyde on reproduction and genetic toxicity of male mice.Methods The male Kunming mice were randomly divided into negative control group (NC),formaldehyde exposed groups (21,42,84 mg·m^-3 )　 and positive control group (PC).12 male mice were included in each group.The exposed dose was respectively 21 mg·m^-3  (1/24LC₅₀),42 mg·m^-3  (1/12 LC₅₀) and 84 mg·m^-3  (1/6 LC₅₀).Mice in negative control group inhaled air,the mice in positive control group were injected with cyclophosphamide.The mice were killed after exposure to toxicant,and the testicle cell apoptosis and cell cycle in mice in various groups were examined.Results The apoptotic rate of mouse testicle cells in 1/6LC₅₀ group was significantly higher than that in negative control (P<0.05).The percentages of S phase cells gradually increased and that of G₀/G₁ and G2+M　phase cells gradually decreased with the formaldehyde dose increasing.The percentages of S-phase cells in three exposed groups were obviously higher than that in negative control and that ofG0/G1 and G2+M　phase cells in 1/6 LC₅₀ group were obviously lower than other groups(P<0.05).Conclusion Formaldehyde can increase the apoptotic rate of mouse testicle cells,and  increase the percentages of S phase cells and decrease the percentages  of G0/G1and G2+M phase cells.The results suggest that formaldehyde has reproductive and genetic toxicity.

Key words: formaldehyde, testicle cell, apoptosis, cell cycle