TGF-β1对人舌鳞状细胞癌上皮-间质转化的诱导作用及其对PI3K/AKT信号通路的影响

doi:10.13481/j.1671-587x.20150423

Abstract

Abstract:

Objective To investigate the effects of transforming growth factor β1 (TGF-β1) on the epithelial-to-mesenchymal transition(EMT)in human tongue squamous cell carcinoma, and to explore its influence in PI3K/AKT signaling pathway. Methods The in vitro cultured tongue squamous cell carcinoma SCC9 cells and CAL27 cells were divided into control group and different concentrations (5 and 10 μg·L^-1)of TGF-β1 groups;24 h after TGF-β1 treatment,the morphology of cells in various groups were observed under light microscope. Immunoflurescence staining was used to detect the expressions of Vimentin and E-cadherin in the cells in various groups. The expressions of AKT and p-AKT in the SCC9 and CAL27 cells were measured by Western blotting method. Results Compared with control group,the SCC9 and CAL27 cells with polygonal morphology trans-differentiated into mesenchymal phenotype with an elongated morphology in TGF-β1 groups.Compared with control group,the expressions of Vimentin protein in the cells in TGF-β1 groups were increased and the expressions of E-cadherin were decreased. Compared with control group,the expression levels of p-AKT were decreased in SCC9 and CAL27 cells in TGF-β1 groups. Conclusion TGF-β1 can induce the EMT in human tongue squamous cell carcinoma and affects the PI3K/AKT signaling pathway.

Key words: transforming growth factor β1, tongue neoplasms, epithelial-to-mesenchymal transition, PI3K/AKT

CLC Number:

R739.8

FU Liangbin, LIAO Tianan, WANG Hong, DENG Wei, ZHOU Bin, WANG Youyuan. Induction effect of TGF-β1 on epithelial-to-mesenchymal transition of human tongue squamous cell carcinoma and its influence in PI3K/AKT signaling pathway[J].Journal of Jilin University Medicine Edition, 2015, 41(04): 790-793.

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Induction effect of TGF-β1 on epithelial-to-mesenchymal transition of human tongue squamous cell carcinoma and its influence in PI3K/AKT signaling pathway

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