五倍子酸对人胃癌SGC-7901细胞迁移能力的影响

doi:10.13481/j.1671-587x.20170415

Abstract

Abstract: Objective: To study the influence of gallic acid (GA) on the migration of human gastric carcinoma SGC-7901 cells, and to explore its mechanism.Methods: The human gastric carcinoma SGC-7901 cells were cultured and divided into control group and 3.125, 6.250, 12.500, 25.000, 50.000,100.000 mg·L^-1 GA groups. The inhibitory rates of proliferation of SGC-7901 cells in various groups were examined by MTT assay; the migration abilities of SGC-7901 cells in various groups were measured with scratch assay; the expression levels of vascular of endothelial growth factor (VEGF) in various groups were detected by immunocytochemistry.Results: Compared with control group, the inhibitory rates of proliferation of SGC-7901 cells in different doses of GA groups were significantly increased in a dose-dependent manner(F=59.451,P<0.01). Compared with control group, the wound healing rates in different doses of GA groups were significantly decreased (P<0.01). Compared with control group, the expression levels of VEGF protein in 12.500 and 25.000 mg·L^-1 GA groups were decreased (P<0.05).Conclusion: GA could inhibit the proliferation and migration of SGC-7901 cells through down-regulating the expression levels of VEGF protein.

Key words: cell migration, vascular endothelial growth factor, gallic acid, SGC-7901 cells

CLC Number:

R285.5

WANG Liping, FENG Ruo, ZOU Liyang, DING Yi. Influence of gallic acid in migration ability of human gastric carcinoma SGC-7901 cells[J].Journal of Jilin University Medicine Edition, 2017, 43(04): 739-742.

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Influence of gallic acid in migration ability of human gastric carcinoma SGC-7901 cells

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