Abstract:

Abstract:Objective
To investigate    the changes of neuronal activity of habenula and the regulatory effect of Hb on   sleep under the condition of short-time sleep deprivation(SD),and to clarify the effects of lateral habenula (LHb) on sleep rebound after short-time SD.Methods 24 adult healthy Wistar rats were randomly divided into control group (n=12) and  SD group (n=12).The model  of SD was set up by small platform surrounded by water.The typical control for this procedure was to maintain the animal on a large platform.The levels of glutamic acid(Glu) and γ-aminobutyric acid(GABA) in Hb neurons were assessed by HPLC-UV detector.The amounts of c-Fos positive neurons were detected by immunohistochemistry.Results Compared with control group,the levels of Glu in Hb was  increased (P<0.05),and the level of GABA had an incrensing trend.The amount of c-Fos positive neurons was increased markedly in   LHb(P<0.05),but not in  medial Hb(MHb).Conclusion The LHb is related to the sleep rebound after SD,but not the MHb.

Key words: sleep deprivation;lateral habenula;glutamic acid;gamma-aminobutyric acid;c-Fos protein