Abstract:

Abstract:Objective
To investigate the inhibitory effect of phenylacetate(PA)  on cell proliferation of pancreatic cancer cells and to study the mechanism of the effect at the molecular level. Methods The inhibitory effects of different doses of PA (0,0.25,0.50,1.00,2.00 and 4.00 mmol•L^-1) on pancreatic cancer cells BXPC-3 were detected by MTT and cell numeration methods.The cell percentages in different cell cycles were tested by flow cytometry(FCM). Results After treated with different concentrations (0.25,0.50,1.00,2.00 and 4.00 mmol•L^-1) of  PA for  72 h,the inhibitory rates of proliferation  of BXPC-3 cells (31.0%±1.6%，54.8%±1.2%，68.3%±2.4%，79.9%±2.1%，81.1%±1.3%,respectively) were much higher than that in  control group(1.2％±0.2%)（P<0.05）.With the increasing of concentrations from 0.25 to 2.00 mmol•L^-1,the inhibitory rates of  proliferation were significantly increased (P<0.05)，but there was no significant difference  between 2.00 and 4.00  mmol•L^-1 PA groups（P>0.05）. The FCM result showed that the ratio of G0/G1 phase of BXPC-3 cells after treated with 1.0 mmol•L-1 PA for  72 h was decreased from 61.8% to 51.3%,the ratio of G2+M phase from 9.4% to 9.2%;the ratio of G0/G1 phase of BXPC-3 cells after treated with 2.0 mmol•L^-1 PA  72 h was decreased from 61.8% to 32.3%,the ratio of G2+M phase from 9.4% to 8.1%，the difference between 2.00 mmol•L-1 PA and control group was  significant（P<0.05）. Conclusion PA can supress the cell cycle  at G1 phase,and decrease the DNA synthesis,and inhibit the cell proliferation. The mechanism is that PA  can inhibit the RNA expressions of some kinds of proteins in G1 phase in BXPC-3 cells.

Key words: phenylacetate;pancreatic neoplasms;cell cycle;DNA;BXPC-3