J4 ›› 2012, Vol. 38 ›› Issue (1): 89-93.
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LU Yang|LI Wei|CUI Jiu-wei|XU Xiao-yi|WANG Guan-jun
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Abstract:
Objective To investigate the influence of juglon on adhesion,activities and expressions of matrix metalloproteinases(MMP2 and MMP9) in human colon carcinoma HCT-8 cells,and clarify the possible mechanism that juglone can inhibit the invation and migration of tumor. Methods The human colon carcinoma HCT-8cells cultivated in vitro at logarithmic growth phase were divided into 1.25,2.50,5.00,10.00 and 20.00 mg.L-1 juglone groups,while the control group was set up. The ability of cell adhesion was detected by fibronectin adhesion assay after treated for 72 h,the activities of MMP2 and MMP9 of the cells were detected by gelatin zymography analysis,the expressions of MMP2 and MMP9 proteins were examined by Western blo tting method. Results Compared with control group, the adhesion rates of HCT-8 in 1.25,2.50,5.00,10.00 and 20.00 mg.L-1juglone groups were decreased(P<0.05);the activities of MMP2 in 2.50,5.00,10.00 and 20.00 mg.L-1 juglone groups was decreased;the activities of MMP9 in all juglone groups with different concentrations were decreased(P<0.05);the expressions of MMP2 and MMP9 proteins in juglone groups with different concentrations were decreased(P<0.01). Conclusion Juglone could significantly inhibit the adhesion ability of HCT-8 cells and decrease the activities and protein expressions of MMP2 and MMP9. It is suggested that the decreasing of the activities and protein expressions of MMP2 and MMP9 might be the possible mechanism that juglone can inhibit the adhesion of HCT-8 cells in vitro.
Key words: juglone;cell adhesion;matrix , metalloproteinases;colon neoplasms
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LU Yang|LI Wei|CUI Jiu-wei|XU Xiao-yi|WANG Guan-jun. Influence of juglone on adhesion and activities of matrix metalloproteinases in human colon carcinoma HCT-8 cells[J].J4, 2012, 38(1): 89-93.
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http://xuebao.jlu.edu.cn/yxb/EN/Y2012/V38/I1/89
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