Abstract:

To study the influence of Rhizoma Typhonii extracts in glioma SHG-44 cells and to explore the effects of Rhizoma Typhonii extracts on proliferation and apoptosis of　glioma cells.Methods Glioma SHG-44 cells were cultured and divided into control and 8,40,200,and 1 000  μg•L^-1 Rhizoma Typhonii extracts groups,and the influence of Rhizoma Typhonii extracts in proliferation of SHG-44 cells was measured by MTT assay.The morphology of apoptosis was observed with inverted microscope.The cell cycle and apoptotic rate were analyzed by flow cytometry (FCM).The expressions of Bcl-2 and Bax protein were detected by immunohistochemistry.Results Compared with control group,the poliferation of SHG-44 cells was inhibited after treated with Rhizoma Typhonii extracts at concentrations of 8 and 200 μg•L^-1 for 30 min and 3 h and the proliferation activities were decreased(P＜0.05);in 1 000 μg•L^-1 group　after treated for  30 min,1 h and 3 h,the proliferation activities were  significantly decreased (P＜0.05).The proliferation inhibition of differentconcentrations of Rhizoma Typhonii extracts was time- and dose-dependent.The apoptotic bodies were found under inverted microscope in different Rhizoma Typhonii extracts groups.The FCM results showed that part of cells were arrested at G2/M phase,and the apoptotic rates were increased with the  increasing of drug concentrations.Cell immunohistochemisty showed that compared with control group the expression of Bcl-2 protein in 200 μg•L^-1 Rhizoma Typhonii extracts groups was decreased(P<0.01) and the expression of Bax protein  was increased (P<0.01).Conclusion Rhizoma Typhonii extracts can inhibit the proliferation and induce apoptosis by down-regulating the Bcl-2 protein expression and up-regulating the Bax protein expression in SHG-44 cells.

Key words: Rhizoma Typhonii, glioma cells, proliferation, apoptosis