贝母瓜蒌散对慢性阻塞性肺疾病大鼠肺组织中血管紧张素Ⅱ表达水平的影响

doi:10.13481/j.1671-587x.20150318

Abstract

Abstract:

Objective To investigate the effects of BeiMuGuaLou powder on the expression of angiotensin Ⅱ(AngⅡ) in thelung tissue of the rats with chronic obstructive pulmonary disease(COPD), and to study the protective effect of BeiMuGuaLou powder on lung tissue and its mechanism. Methods Thirty male SD rats were randomly divided into control group, COPD group and BeiMuGuaLou powder group, 10 rats in each group.The model of COPD was established by smoking combined with endotracheal injection of lipopolysaccharide(LPS) for 30 d.On the 8th day of modeling, the rats in control and COPD groups were lavaged with normal saline(20 mL·kg^-1).The rats in BeiMuGuaLou powder group were lavaged with BeiMuGuaLou powder(20 mL·kg^-1) for 22 d.The expression levels of AngⅡ in the lung tissue of the rats in various groups were determined by immunohistochemistry and Western blotting methods. Results Compared with control group, the rats in model group appeared dyspnea, cough, shortness of breath, apathia, spit saliva, yellow hair, yellow teeth, and other symptoms and signs.Compared with control group, the expression level of AngⅡ in lung tissue of the rats in model group was significantly increased(P<0.05);compared with model group, the expression level of AngⅡ in lung tissue of the rats in BeiMuGuaLou powder group was significantly reduced (P<0.05). Conclusion BeiMuGuaLou powder can resist the cell inflammatory reaction induced by AngⅡ, and obviously improve the asthma and cough, shortness of breath and other symptoms of the COPD rats.

Key words: BeiMuGuaLou powder, chronic obstructive pulmonary disease, angiotensin Ⅱ, rats

CLC Number:

R563

WU Zhonglian, HUANG Xuekuan, LUO Yan, ZHANG Chaonan, JIANG Juan. Effects of BeiMuGuaLou powder on expression level of angiotensin Ⅱ in lung tissue of rats with chronic obstructive pulmonary disease[J].Journal of Jilin University Medicine Edition, 2015, 41(03): 527-531.

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Effects of BeiMuGuaLou powder on expression level of angiotensin Ⅱ in lung tissue of rats with chronic obstructive pulmonary disease

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