Abstract:

Objective To study the correlation between the expression of Galectin-3 and Bcl-2,Mmp-2 and K-ras in laryngeal squamous carcinoma(LSCC) and the relationship between Galetin-3 expression and  clinicopathological characteristics.Methods The expressions of Galectin-3,Bcl-2,Mmp-2 and K-ras  in LSCC tissues of 32 patients,normal mucosa tissues of 32 cases,and polyp of vocal cord tissues of 10 cases were detected by immunohistochemistry staining.The relationship between  their expressions and the pathological characteristics of LSCC,clinical staging and lymph node metastasis in LSCC was analyzed.The correlation between the expression of Galectin-3 in LSCC tissue and Bcl-2,Mmp-2 and K-ras expressions in LSCC tissue was analyzed.Results The differences in the  expressions of Galectin-3,Bcl-2,Mmp-2 and K-ras between  normal mucosa tissues,polyp of vocal cord tissues and LSCC tissues were significant  （P<0.05）.The expressions of Galectin-3 and Bcl-2 were related to pathological grade of LSCC（P<0.05）,the expression of Bcl-2 was related to clinical stages and lymph node metastasis of LSCC（P<0.05）.The expression of Galectin-3 had positive correlation with  Bcl-2,Mmp-2, and K-ras expressions in LSCC tissue(r₁＝0.993，r_２＝0.989，r_３＝0.992).Conclusion The high expressions of Galectin-3,Mmp-2 and K-ras are closely related to the occurrence and development of the LSCC.The expression of Galectin-3 is positively related to the expressions of Bcl-2,Mmp-2 and K-ras.Co-expression of Galectin-3,Bcl-2,Mmp-2 and K-ras are able to promote the pathogenesis of LSCC.Galectin-3 is possible to become a new target gene of LSCC gene theray.

Key words: laryngeal neoplasm, Galectin-3, Bcl-2, Mmp-2, K-ras； immunohistochemistry