Abstract:

Abstract:Objective
To explore the effects of double interfering vector pGenesil-2.1-PTTG-survivin siRNA  on PTTG and survivin gene expressions in human U251 glioma cell line. Methods U251 cells  were divided in to five groups: normal control,negative control (Lipo2000+ pGenesil-2.1 HK),Lipo2000+pGenesil-2.1-PTTG siRNA,Lipo2000+ pGenesil-2.1- survivin siRNA and Lipo2000+pGenesil-2.1-PTTG-survivin siRNA groups.The  PTTG and survivin protein and mRNA levels in every group were measured with flow cytometry,Western blotting and RT-PCR method after U251 cells were transfected with these plasmids. Results The　 PTTG and survivin protein and mRNA levels in Lipo2000+pGenesil-2.1 PTTG siRNA,Lipo2000+ pGenesil-2.1 survivin siRNA and Lipo2000+pGenesil-2.1-PTTG-survivin siRNA groups were decreased significantly compared with negative control group（P<0.01）,especially in pGenesil-2.1-PTTG-survivin siRNA group. Conclusion The silence effects of   double interfering vector pGenesil-2.1-PTTG-survivin siRNA on PTTG and survivin gene in U251 cells are stronger than those of  pGenesil-2.1-PTTG siRNA or pGenesil-2.1- survivin siRNA.

Key words: pituitary tumor transforming gene;survivin;RNAi;glioma