RNA干扰YWHAZ基因对结肠癌HCT-8细胞增殖的影响

doi:10.13481/j.1671-587x.20150220

Abstract

Abstract:

Objective To investigate the effect of RNA interference of YWHAZ gene on the cell proliferation of colon cancer HCT-8 cells,and to elucidate the mechanism.Methods Four siRNA plasmids targeting human YWHAZ gene,including pGPU6/GFP/Neo-YWHAZ-792,pGPU6/GFP/Neo-YWHAZ-733,pGPU6/GFP/Neo-YWHAZ-612,and pGPU6/GFP/Neo-YWHAZ-505, were constructed and regarded as RNA interference plasmid groups. pGPU6/GFP/Neo-shNC plasmid was regarded as negative control plasmid group. RT-PCR was used to detect the expression of YWHAZ mRNA and the inhibitory rates of HCT-8 cells; the proliferation rate of HCT-8 cells was detected by MTT method.Results The transfection rate of cells was 60% under inverted fluorescence microscope;the RT-PCR results showed that the levels of YWHAZ mRNA in RNA interference plasmid groups were lower than those in negative control plasmid group(P<0.01),and the average inhibitory rate was 52.86%. The MTT results showed that compared with negative control plasmid group,the proliferation rates of HCT-8 cells in RNA interference plasmid groups were decreased (P<0.01),and the average proliferation rate was 68.75%.Conclusion Interference of YWHAZ gene might inhibit the transcription of the gene,and further inhibit the proliferation of HCT-8 cells.

Key words: YWHAZ gene, colon neoplasms, HCT-8 cells, RNA interference

CLC Number:

R735.3

ZHANG Chengcai, SONG Yanyan, ZHOU Mengjiao, CUI Zhiqian, ZHANG Xichen, XING Shenyang. Effect of RNA interference of YWHAZ gene on proliferation of colon cancer HCT-8 cells[J].Journal of Jilin University Medicine Edition, 2015, 41(02): 307-310.

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Effect of RNA interference of YWHAZ gene on proliferation of colon cancer HCT-8 cells

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