五味子乙素通过p38MAPK信号通路对结肠癌SW480细胞凋亡和侵袭的影响

doi:10.13481/j.1671-587x.20150401

Abstract

Abstract:

Objective To investigate the roles of schisandrin B (SchB) on the apoptosis and invasion of human colon cancer SW480 cells and its influence in p38MAPK signaling pathway,and to clamify the mechanisms. Methods The SW480 cells were divided into control group,low dose of SchB group (20 μmol·L^-1,SchB₁ group),middle dose of SchB group (40 μmol·L^-1,SchB₂ group) and high dose of SchB group (80 μmol·L^-1,SchB₃ group).After the SW480 cells were treated with different doses of SchB,the inhibitory rates of proliferation of the SW480 cells were evaluated using MTT assay,the apoptotic rates were analyzed by flow cytometry using Annexin V-FITC and PI double staining methods,the invasion activities of SW480 cells were tested using Transwell chamber,and the expression levels of p-p38,p38,p-p53 and p53 were measured by Western blotting method. Results Compared with control group,the inhibitory rates of proliferation and the apoptotic rates of colon cancer SW480 cells in SchB₁,SchB₂ and SchB₃ groups were increased(P<0.01),the invasion rates and the number of SW480 cells penetrating the membrane were decreased (P<0.01), and the expression levels of p-p38 and p-p53 proteins were increased (P<0.05 or P<0.01). Conclusion SchB can inhibit the proliferation and invasion of human colon cancer SW480 cells and promote the apoptosis.p38MAPK signaling pathway may be involved in the inhibitory effect of SchB on the tumor cells.

Key words: schisandrin B, colon neoplasms, p38 mitogen activated protein kinases

CLC Number:

R285.5

JIANG Enping, LI He, YU Chunyan, ZHU Wei. Influence of schisandrin B in apoptosis and invasion of SW480 cells via p38MAPK signaling pathway[J].Journal of Jilin University Medicine Edition, 2015, 41(04): 675-679.

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Influence of schisandrin B in apoptosis and invasion of SW480 cells via p38MAPK signaling pathway

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