银杏叶提取物对大鼠骨髓间充质干细胞成脂分化能力的影响

doi:10.13481/j.1671-587x.20150227

Abstract

Abstract:

Objective To study the effect of Ginkgo biloba extract (GBE) on the adipogenic differentiation ability of the bone marrow mesenchymal stem cells(BMMSCs),and to clarify its significance in treatment and prevention of osteoporosis at the cell level.Methods The third generation of BMMSCs were cultured with adipogenic induction solution(control group),and 50,100,150,200, and 400 mg·L^-1 GBE(50,100,150,200, and 400 mg·L^-1 GBE groups) for 7 d.The morphology of BMMSCs and the expressions of surface antisgens CD44,CD105, and CD34 were observed;the formation of lipid droplet in the BMMSCs was observed by Oil Red O staining;the quantitative analysis of the adipogenic ability of the BMMSCs was performed;RT-PCR method was used to test the expression levels of fatty acid-binding protein(AP2) and peroxisome proliferator activated receptor-γ(PPAR-γ) mRNA.Results The morphological detection results showed that the BMMSCs were spindle shaped after cultured in vitro for 3 d,and grew in whorls and arranged in the same direction after cultured for 7 d.The surface antigens CD44 and CD105 showed positive expression,and CD34 showed negative expression,which proved the BMMSCs were extracted correctly;the Oil red O staining results showed a large of lipid droplets which was seen under microscope,and the number of lipid droplets was reduced with the increasing of the concentrations of GBE; compared with control group,the A₅₁₀ values of the BMMSCs in other groups were decreased(P<0.05).The RT-PCR results showed the expression levels of AP2 and PPAR-γ mRNA were decreasd in other groups compared with control group (P<0.05).Conclusion GBE can inhibit the adipogenic differentiation ability of BMMSCs in vitro,and the inhibitory ability is enhanced with the increasing of GBE concentration.

Key words: Ginkgo biloba extract, bone marrow mesenchymal stem cells, adipogenic differentiation, osteoporpsis

CLC Number:

R914

GU Xu, FANG Hongman, SHI Shuman, ZHANG Jiadi, WU Zhe. Effect of Ginkgo biloba extract on adipogenic differentiation ability of bone marrow mesenchymal stem cells in rats[J].Journal of Jilin University Medicine Edition, 2015, 41(02): 343-346.

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Effect of Ginkgo biloba extract on adipogenic differentiation ability of bone marrow mesenchymal stem cells in rats

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