Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (02): 338-342.doi: 10.13481/j.1671-587x.20150226

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Effects of urinary kallidinogenase on expression levels of IGF-1 and IGF-1R in brain tissue of rats with cerebral ischemia reperfusion and their mechanisms

WANG Jiehua, LI Guoqian, YANG Xiaoxia, HONG Zhuquan   

  1. Department of Neurology, Quanzhou First Affiliated Hospital, Fujian Medical College, Quanzhou 362000, China
  • Received:2014-05-22 Online:2015-03-28 Published:2015-04-04

Abstract:

Objective To observe the effect of urinary kallidinogenase on the expressions of insulin-like growth factor 1(IGF-1) and insulin-like growth factor 1 receptor(IGF-1R) after ischemia reperfusion injury in the rats, and to clarify the mechanism of brain protection.Methods 24 adult SD rats were randomly divided into sham operation group(n=8), model group(n=8),and experiment group(n=8).The middle cerebral artery occlusion reperfusion models were made by the suture method for the rats in model and experiment groups.The rats in sham operation group were taken the similar operation without suture.The rats in model group were given urinary kallilidinogenase 5 min after the reperfusion.The expressions of IGF-1 and IGF-1R mRNA and proteins,and the number of IGF-1 and IGF-1R positive cells in the ischemic region of the brain tissue of the rats in various groups were detected by RT-PCR,immunohistochemical,and Western blotting methods.Results Compared with sham operation group,the expression levels of IGF-1 and IGF-1R mRNA and proteinsand the number of positive cells in the brain tissue of the rats in model group and experiment group were increased (P<0.05);compared with model group,the expression levels of IGF-1 and IGF-1R mRNA and proteins and the number of positive cells in the brain tissue of the rats in experimental group were increased(P<0.05).Conclusion Urinary kallidinogenase can improve the cerebral ischemia reperfusion,which is associated with the promotion of the expressions of IGF-1 and IGF-1R.

Key words: urinary kallidinogenase, cerebral ischemia/reperfusion, insulin-like growth factor 1, insulin-like growth factor 1 receptor

CLC Number: 

  • R541.4