Abstract:

Objective: To study the protective effect of Hawthorn Leaves Flavonoids (HLF)on the experimental myocardial infarction in the dogs,and to clarify its mechanism.Methods: Thirty-six healthy dogs were randomly divided into sham operation group,model group (given the same volume of 0.9% saline),Compound Danshen Injection group (1.65 g·kg^-1),and low,medium and high doses of HLF groups (5,10,20 mg·kg^-1),(n=6);intravenous injection was performed. The method of ligating the anterior descending coronary artery was used to establish acute myocardial infraction models of dogs after anesthesia. The epicardial electrical diagram at 30,60,90,120,180,240,300,360 min of the dogs in various groups afer administration were recorded. The activities of serum aspartate aminotransferase (AST),creatine phosphokinase (CPK),lactate dehydrogenase (LDH),superoxide dismutase (SOD)and malondialdehyde (MDA)levels of the dogs in various groups were detected,and the extent of myocardial ischemia (ΣST)and myocardial infarct size (MIS)were calculated. Results: Compared with sham operation group,the serum AST,CPK,LDH activities and MDA level of the dogs in model group were increased (P<0.05),the SOD activity was decreased (P<0.05),and the whole heart weight percentage,ventricular weight percentage and ∑ST were increased (P<0.05 or P<0.01).Compared with model group,the serum AST,CPK,LDH activities and MDA levels of the dogs in medium and high doses of HLF groups were decreased (P<0.05 or P<0.01),the SOD activities were increased (P<0.05 or P<0.01),and the ΣST were decreased (P<0.05 or P<0.01);the whole heart and ventricular weight percentages of the dogs in low,medium,and high doses of HLF groups were decreased (P<0.05 or P<0.01). Conclusion: Hawthorn Leaves Flavonoids has the protective effect on experimental myocardial infarction in the dogs.

Key words: Hawthorn Leaves Flavonoids, myocardial infarction, myocardial enzymes, electrocardiogram