Abstract:

Abstract:Objective To explore the effects of atorvastatin(AT) on myocardial enzymes and apoptosis of myocardial cells in rats with myocardial ischemia reperfusion (I/R) injury and to clarify the mechanism of protective effect of AT on I/R rat myocardium tissue.Methods 80 healthy male Wistar rats,3 months old,were randomly divided into  sham operation(S) group,ischemia reperfusion(I/R) group,large dose AT group (LAT group,10 mg/kg) and small dose AT group (SAT group,1 mg/kg)(n=20).I/R model was prepared by ligating the coronary artery of the left anterior descending artery(LAD).The biochemical methods were used to detect the contents of serum creatine kinase(CK),lactate dehydrogenase(LDH),malondialdehyde(MDA),nitric oxide(NO),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and glutathione(GSH) in myocardium tissue;the immunohistochemical methods were used to detect the expressions of Fas,Bax and Bcl-2 proteins in myocardium tissue.Results Compared with S group,the  myocardial CK,LDH,MAD activities of the rats in I/R group were increased(P<0.01), and the NO,SOD,GSH-Px,GSH activities were decreased(P<0.01).Compared with I/R group,the CK activities,LDH activities and serum MDA activities  in LAT and SAT groups were reduced (P<0.01);and the levels of serum NO,serum SOD activities,GSH-Px activities and GSH contents were increased(P<0.01),but there was no significant difference between LAT group and SAT group (P>0.05).Compared with S group, the Fas and of Bax expressions in myocardium tissue in I/R group,LAT group and SAT group were significantly increased (P<0.01).Compared with  I/R group,the Fas  protein expression in rat myocardium tissue in LAT and SAT groups were significantly reduced(P<0.01);but the expression of Bax  protein had no significant difference between LAT group,SAT group and I/R group (P>0.05).Compared with S group,the Bcl-2  protein expressions in I/R group,LAT group,and SAT group were increased,and there was significant difference between LAT group and S group (P<0.01),while the difference was not statistically significant(P>0.05) among the other groups.Conclusion AT has protective effect on myocardium tissue of the rats with myocardial  I/R injury,and its mechanism may be related to reducing the changes of myocardia enzymes and inhibiting the protein expression of Fas  and promoting the expression of Bcl-2  protein.

Key words: myocardial ischemia reperfusion, atorvastatin, myocardial enzymes, apolipoprotein, Bcl-2 protein, Bax protein