NRP1和miR-9在人NSCLC组织和癌旁组织中的表达及其临床意义

doi:10.13481/j.1671-587x.20160219

Abstract

Abstract:

Objective: To detect the expression levels of neuropilin1(NRP1) mRNA and miR-9 in non-small cell lung cancer (NSCLC) tissue samples, and to explore the correlations between the expressions of NRP1 mRNA, miR-9 and the clinicopathological characteristics of the patients with NSCLC. Methods: Informed consent was obtained from each patient before surgery.The tissue samples including 45 NSCLC tissue, 45 adjacent carcinoma tissue and 45 normal lung tissue were collected from China-Japan Union Hospital of Jilin University from 2010 to 2011. qRT-PCR was used to detect the expression levels of NRP1 mRNA and miR-9 in three kinds of lung tissue, and the correlation between the expressions of NRP1 mRNA, miR-9 and clinicopathological characteristics of the patients with NSCLC was analyzed.Results: Compared with normal tissue, the expression level of NRP1 mRNA in adjacent carcinoma tissue had no change(P>0.05), but the expression level of NRP1 mRNA in non-small cell lung cancer tissue was significantly decreased (P<0.05).Compared with normal tissue, the expression level of miR-9 in adjacent carcinoma tissue had no change(P>0.05), but the expression level of miR-9 in non-small cell lung cancer tissue was significantly increased (P<0.05).Furthermore, in adjacent carcinoma tissue, the expression level of miR-9 in the males was lower than that in the females(P<0.05).In NSCLC tissue, the expression level of NRP1 mRNA had no relationship with sex, age, differentiation degree, TNM stage and clinical stage, but was significantly correlated to the histological subtype and lymph node metastasis (P<0.05). In NSCLC tissue, the expression level of miR-9 had no relationship with age, pathological type, lymph node metastasis, differentiation degree, TNM stage, and clinical stage (P>0.05), but was correlated to the sex(P<0.05).Conclusion: The expression level of miR-9 is up-regulated and the expression level of NRP1 mRNA is down-regulated significantly in non-small cell lung cancer tissue.The detection of the expression level of NRP1 mRNA contributes to judge the histological subtype and lymph node metastasis of NSCLC.

Key words: neuropilin1, miR-9, carcinoma, non-small cell lung, clinicopathological characteristics

CLC Number:

R734.2

JIN Linlin, SHAO Lihong, WEI Wei, DONG Zhuo, YUAN Tianyang, GAO Hui, HAN Hailing, CONG Xianling, JIN Shunzi. Expressions of miR-9 and NRP1 in human NSCLC tissue and adjacent carcinoma tissueand their clinical significances[J].Journal of Jilin University Medicine Edition, 2016, 42(02): 290-294.

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Expressions of miR-9 and NRP1 in human NSCLC tissue and adjacent carcinoma tissueand their clinical significances

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