Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (05): 932-936.doi: 10.13481/j.1671-587x.20160518

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Expression of Disabled-1 in human breast cancer cells and its role in cell cycle

CAO Rangjuan1, LI Kai2, XING Wanying3, WANG Yueshu1, YU Wei1, WU Guangzhi1, CUI Shusen1, LI Qiang1   

  1. 1. Department of Hand Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033;
    2. Department of Anesthesiology, China-Japan Union Hospital, Jilin University, Changchun 130033;
    3. Department of Breast Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China
  • Received:2016-04-12 Online:2016-09-28 Published:2016-09-29

Abstract:

Objective: To explore the expressions of Disabled-1(Dab1) in human breast epithelial cells and breast cancer cells, and to clarify its role in cell cycle. Methods: Real-time PCR was used to analyze the Dab1 mRNA expressions in breast epithelial cells MCF-10A and breast cancer cells MCF-7, BT-549, and MDA-MB-231. The Dab1 protein expressions in those cells were tested by Western blotting method. The BT-549 cells at logarithmic growth phase were divided into control, pKH3, and pKH3-Dab1 groups; the cell cycle was investigated by flow cytometry. Results:The Real-time PCR results showed that the Dab1 mRNA expression levels in MCF-7 cells (0.504±0.037), BT-549 cells (0.302±0.027), and MDA-MB-231 cells (0.330±0.031) were reduced compared with MCF-10A cells (0.998±0.020) (P<0.05). The Western blotting results showed that the Dab1 protein expression levels in breast cancer cells MCF-7 (0.134±0.014), BT-549 (0.076±0.01), and MDA-MB-231 (0.074±0.005) were reduced compared with MCF-10A cells (0.227±0.021) (P<0.05). Compared with control group and pKH3 group, the cell cycle in pKH3-Dab1 group was inhibited at G1 phase detected by flow cytometry analysis. Conclusion: The expression of Dab1 is down-regulated in breast cancer cells, and the over-expression of Dab1 can inhibit the cell cycle at G1 phase.

Key words: Dab1, human breast cancer cell, cell cycle, G1 phase inhibition

CLC Number: 

  • R730.2