单宁酸对高糖下大鼠肾小球系膜细胞增殖及细胞周期的影响

doi:10.13481/j.1671-587x.20160516

Abstract

Abstract:

Objective: To explore the inhibitory effect of gallotannin (GLTN) on the proliferation of rat glomerular mesangial cells (GMC) induced by high sugar stimulation and the influence in the cell cycle of the rats,and to clarify the prevention effect of GLTN in the occurrence and development of diabetic nephropathy (DN). Methods: The experimental cells were divided into normal control group (D-glucose 5.5 mmol·L^-1, NC group), high glucose group (D-glucose 30 mmol·L^-1, HC group), high glucose+5 mmol·L^-1 3-AB group (AB group), high glucose+20μmol·L^-1 GLTN group (G20 group), high glucose+40 μmol·L^-1 GLTN group (G40 group).The proliferation of GMC in different groups at different time points(4,8,24,48 and 72 h)was observed by MTT assay. The changes of cell cycle of GCM under different culture conditions were examined by flow cytometry, and the expression levels of TGF-β1 and CTGF were detected by Western blotting method. Results: Compared with NC group, the proliferation levels of GMC in HC group were increased (P<0.01), and reached the peak at 48 h; the percentage of S phase cells was increased(P<0.01). Compared with HC group, the proliferation levels of GMC in 3-AB group and GLTN group were significantly decreased (P<0.01), and the percentages of S phase cells were decreased (P<0.01). Compared with NC group, the protein expression levels of TGF-β1 and CTGF in each drug group were increased (P<0.05 or P<0.01), but they were significantly lower than those in HC group (P<0.01). Conclusion: GLTN can inhibit the proliferation of GMC under high sugar stimulation through arresting the cell cycle and down-regulating the expressions of TGF-β1 and CTGF and delay the occurrence and development of DN.

Key words: gallotannin, diabetic nephropathy, cell proliferation, cell cycle, trasforming growth factor-β1, connective tissue growth factor

CLC Number:

R587

QU Meng, JIANG Rui, FENG Kai, SUN Liwei, DONG Zhiheng. Effects of gallotannin on proliferation and cell cycle of rat glomerular mesangial cells under condition of high glucose[J].Journal of Jilin University Medicine Edition, 2016, 42(05): 920-924.

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Effects of gallotannin on proliferation and cell cycle of rat glomerular mesangial cells under condition of high glucose

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