Abstract:

Abstract:Objective To investigate the influence and effect of rAV-Tumstatin on T24 cell lines,and provide experimental basis　for further  study on its   mechanism.Methods rAV-Tumstatin　was constructed and the T24 cell lines were infected.The cells were divided into rAV-Tumstatin group,vacant viral infection group and control group.The proliferation and apoptosis of the infected cells were detected  by  MTT and TUNEL.Results The T24 cells  could  be stably  infected  by  rAV-Tumstatin and   expressed the exogenous gene EGFP. The result of TUNEL showed the apoptotic rates in rAV-Tumstatin  group,vacant viral infection group and control groupwere 32.9%,13.5%,and 6.7%,respectively;there were significant differences between  three groups(P<0.01).The result of MTT which detected the proliferation and growth of T24 cells respectively  showed  as  follows: rAV-Tumstatin group,G0/G1 60.3%,S 17.4%,G2/M 21.5%;vacant viral infection group,G0/G1 45.2%,S 29.5%,G2/M 24.8%; control group,G0/G1 43.7%,S 32.8%,G2/M 23.7%;there 　were also 　significant differences between three groups(P<0.05).Conclusion rAV-Tumstatin can inhibit the proliferation and induce the apoptosis of T24 cells,and it is very valuable for the gene therapy of bladder cancer.

Key words: bladder neoplasms;recombinant adenovirus;Tumstatin