J4 ›› 2010, Vol. 36 ›› Issue (3): 450-452.
Previous Articles Next Articles
ZHANG Ming, WEI Wei, LIU Lu-Cheng, LI Zhi, WEN Dou-Su
Received:
Online:
Published:
Abstract:
Abstract:Objective To investigate the effect of miRNA-21 antisense oligonucleotide(ASOND) on the proliferation and apoptosis of bladder cancer cell line T24,and provide theoretical basis for treatment of bladder cancer. Methods AS-miRNA-21 mediated by liposome was transfected into T24 cells. As-miRNA-21 group,transfection nonsense series group and control group were set up.The protein expression level of miRNA-21 of the transfected cells was detected by Western blotting,and the proliferation and apoptosis of the transfected cells were measured by MTT and FCM.Results The miRNA-21 expression level of the tumor cells transfected with AS-miRNA-21 was decreased,the cell proliferation was inhibited.The proliferation rates of T24 cells in AS-miRNA-21 group,transfection nonsense series group and control group were 53.6%,92.5%,and 100%,respectively; the relative apoptotic rates were 13.8%,1.9% and 2.7%.There were significant differences of the proliferation rate and apoptotic rates between the three groups(P<0.01).Conclusion Antisense miRNA-21 has the action of proliferation inhibition and apoptosis enhancement on human bladder cancer cell T24. Inhibiting the expression of miRNA-21 may become a prospective gene therapy for bladder cancer.
Key words: bladder neoplasms;miRNA-21;antisense oligonucleotides
CLC Number:
ZHANG Ming, WEI Wei, LIU Lu-Cheng, LI Zhi, WEN Dou-Su. Effect of antisense miRNA-21 on proliferation and apoptosis of bladder cancer cells[J].J4, 2010, 36(3): 450-452.
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://xuebao.jlu.edu.cn/yxb/EN/
http://xuebao.jlu.edu.cn/yxb/EN/Y2010/V36/I3/450
Cited