miRNA-21对乳腺癌细胞放射敏感性的影响及其机制

doi:10.13481/j.1671-587x.20190405

Abstract

Abstract: Objective:To observe the influence of miRNA-21 in the radiosensitivity of the breast cancer cells, and to preliminarily explore its mechanism. Methods:The breast cancer T47D and MDA-MB-361 cells were irradiated with 0.0,2.5,and 5.0 Gy γ-ray,respectively; CCK8 assay was used to detect the survival rates of T47D and MDA-MB-361 cells and flow cytometry was used to analyze the cell cycle; real-time quantitative PCR was used to detect the expression levels of miRNA-21 in cells during 72 h. The T47D and MDA-MB-361 cells were transfected with anti-miRNA-21 sequence (anti-miRNA-21 group) and negative control sequence(negative control group), respectively; the untransfected cells were set as blank control group. Real-time quantitative PCR was used to detect the expression levels of miRNA-21 in cells in various groups. After irradiation with 0.0 and 5.0 Gy γ-ray, CCK8 assay was used to detect the survival rates, and flow cytometry was used to analyze the cell cycle. Results:After irradiation with 5.0 Gy γ-ray, the survival rate of T47D cells was significantly higher than that of the MDA-MB-361 cells (P<0.05). Compared with 0.0 Gy radiation group, the percentages of T47D and MDA-MB-361 cells in G₂/M phase in 5.0 Gy radiation group were significantly increased(P<0.05); the miRNA-21 expression levels in the T47D and MDA-MB-361 cells were significantly increased (P<0.05).The miRNA-21 expression levels in the T47D and MDA-MB-361 cells in anti-miRNA-21 group were lower than those in blank control group (P<0.05). After irradiation with 5.0 Gy γ-ray, the survival rates of T47D and MDA-MB-361 cells in anti-miRNA-21 group were significantly lower than those in blank control group(P<0.05 or P<0.01), and the percentage of T47D cells in G₂/M phase in anti-miRNA-21 group was significantly lower than that in blank control group (P<0.05), and the percentage of T47D cells in subG₁ phase was significantly higher than that in blank control group (P<0.05). Conclusion:The down-regulation of miRNA-21 may enhance the radiosensitivity of breast cancer cells, and its mechanism may be related to the inhibition of G₂/M phase arrest.

Key words: miRNA-21, breast neoplasms, radiosensitivity, cell survival rate, G₂/M phase arrest

CLC Number:

R737.9

XIN Rui, LI Dong, XU Huiying, WANG Chunyu, QU Danhua, SUN Haifeng. Influence of miRNA-21 in radiosensitivity of breast cancer cells and its mechanism[J].Journal of Jilin University(Medicine Edition), 2019, 45(04): 772-778.

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Influence of miRNA-21 in radiosensitivity of breast cancer cells and its mechanism

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