低剂量电离辐射对STZ诱导糖尿病模型大鼠海马神经元的影响

doi:10.13481/j.1671-587x.20210101

Abstract

Abstract: Objective

To investigate the effects of low dose ionizing radiation （LDR） on the cell cycle， calcium ion concentration（［Ca²⁺］i） and mitochondrial membrane potential（MMP） of hippocampal neurons in the diabetic rats， and to elucidate the protective mechanisms of LDR on nerve injury.

Methods

The Wistar rat models of diabetes mellitus （DM） were established with streptococci （STZ）.Sixty rats were divided into normal control group， DM X-ray irradiation group， DM+25 mGy X-ray irradiation group， DM+50 mGy X-ray irradiation group and DM+75 mGy X-ray irradiation group； there were 12 rats in each group. Except normal control and DM groups， the rats in other groups were administered with 25， 50 and 75 mGy LDR every other day for 12 times， and the body weights of the rats were monitored. After 4 weeks of feeding， the rats were sacrificed after anesthesia. The hippocumpus tissue of the rats in various groups was removed and single cell suspension was prepared. The percentages of hippocampal neurons at different cell cycles，（［Ca²⁺］i） and MMP were detected by flow cytometry， respectively.

Results

With the prolongation of time，the body weight of the rats in normal control group was increased gradually. Compared with normal control group， the body weights of diabetic rats in DM group， DM+25 mGy X-ray irradiation group， DM+50 mGy X-ray irradiation group and DM+75 mGy X-ray irradiation group were significantly decreased （P<0.05）； compared with DM group， the body weights in DM+50 mGy group and DM+75 group mGy group were significantly increased（P<0.05）. Compared with normal control group， the percentages of hippocampal neurons in S phase and ［Ca²⁺］i of the rats in DM group were increased significantly（P<0.05）， while MMP was decreased significantly （P<0.05）； compared with DM group， the percentages of hippocampal neurons in S phase and ［Ca²⁺］i of the rats in DM+50 mGy X-ray irradiation group and DM+75 mGy X-ray irradiation group were decreased significantly （P<0.05）， while MMP of hippocampal neurons was increased significantly （P<0.05）； compared with DM + 25 mGy X-ray irradiation group， the ［Ca²⁺］i in hippocampal neurons in DM+75 mGy X-ray irratiaiton group was decreased significantly（P<0.05）， while MMP of hippocampal neurons in DM+50 Gy X-ray irradiation group and DM+75 Gy X-ray irradiation group were increased significantly （P<0.05）.

Conclusion

LDR can improve the S phase delay， the increase of ［Ca²⁺］i and the decrease of MMP induced by DM of the hippocampal neurons of the rats to play the neuroprotective roles.

Key words: low dose radiation, diabetes mellitus, hippocampal neurons, cell cycle, mitochondrial membrane potential

CLC Number:

Q691

Fei LIU,Shuo LIANG,Yuexuan WANG,Lijing QIN,Wei GUO,Zhicheng WANG. Effect of low dose ionizing radiation on hippocampal neurons in STZ-induced diabetic rats[J].Journal of Jilin University(Medicine Edition), 2021, 47(1): 1-7.

Figures/Tables 5

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Fig. 2

Tab.3

References 22

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Group	Body weight
Group	Before LDR	2 weeks of LDR	4 weeks of LDR	4 weeks after LDR
Normal control	211.0±10.7	260.8±10.5	324.0±19.1	524.0±15.1
DM	207.2±18.3	209.2±2.7^*	212.2±7.7^*	215.2±1.7^*
DM + 25 mGy X-ray irradiation	204.0±11.4	206.9±1.6^*	216.2±4.4^*	223.2±4.4^*
DM + 50 mGy X- ray irradiation	204.6±10.4	212.5±1.9^*	224.0±5.4^*	234.0±28.4^*△
DM + 75 mGy X- ray irradiation	199.2±7.3	209.5±2.7^*	223.8±5.8^*	238.8±5.8^*△

Group	Percentage of hippocampal neurons
Group	G₀/G₁	S	G₂/M
Normal control	87.6±3.8	3.9±0.4	8.6±0.6
DM	85.4±0.9	6.3±0.3^*	8.4±1.0
DM + 25 mGy X-ray irradiation	87.7±2.6	4.7±0.8^*△	7.6±1.4
DM + 50 mGy X-ray irradiation	87.2±0.8	3.5±0.4^*△	9.7±0.9
DM + 75 mGy X-ray irradiation	86.4±2.5	4.5±0.6^*△	9.1±0.9

Group	[Ca²⁺] i	MMP
Normal control	9.4±0.9	31.3±4.3
DM	14.7±1.5^*	25.1±1.4^*
DM+ 25 mGy X-ray irradiation	14.8±0.5^*	25.8±1.0^*
DM+ 50 mGy X-ray irradiation	12.2±0.6^*△	30.5±1.6^△#
DM+ 75 mGy X-ray irradiation	6.5±0.4^*△#	29.1±0.1^△#

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Effect of low dose ionizing radiation on hippocampal neurons in STZ-induced diabetic rats

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