人参果多糖对人舌鳞癌CAL27细胞增殖的抑制作用及其机制

doi:10.13481/j.1671-587x.20200207

Abstract

Abstract: Objective: To investigate the inhibitory effect of polysaccharides from panax ginseng fruit in the human tongue squamous cell carcinoma CAL27 cells,and to elucidate its potential mechanism. Methods: Water soluble ginseng berry polysaccharide (WGBP) was extracted by water extraction and alcohol precipitation method, and its monosaccharide components were analyzed by high performance liquid chromatography (HPLC). The human tongue squamous cell carcinoma CAL27 cells were divided into control group and different concentrations (1,2 and 5 g·L^-1) of WGBP groups. After culture for 24 h, CCK-8 method was used to detect the proliferation rates of the CAL27 cells in various groups, flow cytometry was used to detect the cell cycle and apoptotic rates of the CAL27 cells in various groups,and Western blotting method was used to detect the expression amounts of apoptosis-related proteins in the CAL27 cells in various groups. Results: WGBP was mainly composed of galactose and galacturonic acid, followed by arabinose, glucose, rhamnose and mannose. Compared with control group, the proliferation rates of CAL27 cells in 1,2 and 5 g·L^-1 WGBP groups were increased (P<0.05 or P<0.01) in a concentration-dependent manner.The flow cytometry results showed that compared with control group, the percentages of CAL27 cells in S phase in 2 and 5 g·L^-1 WGBP groups were decreased (P<0.05 or P<0.01), and the percentages of CAL27 cells in G₂/M phase were increased (P<0.05 or P<0.01) in a concentration-dependent manner.The Western blotting results showed that compared with control group, the apoptotic rates of CAL27 cells in 1,2 and 5 g·L^-1 WGBP groups were increased (P<0.05) in a concentration-dependent manner. Compared with control group, the expression amounts of pro-Caspase-3 and pro-PARP proteins in the CAL27 cells in 1,2 and 5 g·L^-1 WGBP groups were increased. Conclusion: WGBP can induce the apoptosis and inhibit the proliferation of human tongue squamous cell carcinoma CAL27 cells by inducing the G₂/M phase arrest and Caspase-3 activation.

Key words: water-soluble ginseng berry polysaccharide, tongue squamous cell carcinoma, cell cycle, apoptosis, galactose

CLC Number:

R739.86

WANG Huiyu, ZHANG Tianfu, HAO Miao, ZHANG Chu, WANG Xiaofeng. Inhibitory effect of polysaccharides from panax ginseng fruit on proliferation of human lingual squamous cell carcinoma CAL27 cells and its mechanism[J].Journal of Jilin University(Medicine Edition), 2020, 46(02): 248-253.

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Inhibitory effect of polysaccharides from panax ginseng fruit on proliferation of human lingual squamous cell carcinoma CAL27 cells and its mechanism

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