转染TRIM29-siRNA对胶质瘤U87MG细胞周期和凋亡的影响

doi:10.13481/j.1671-587x.20200219

Abstract

Abstract: Objective: To investigate the effects of tripartitemotif containing 29(TRIM29)-siRNA transfection on the cell cycle and apoptosis of glioma U87MG cells,and to elucidate the role of TRIM29 in the occurrence and development of glioma. Methods: The U87MG cells cultured in vitro were divided into control group (untransfected), NC-siRNA group (transfected with negative control NC-siRNA), and TRIM29-siRNA group (transfected with TRIM29-siRNA). The expression levels of TRIM29 mRNA in the U87MG cells in three groups were detected by real-time fluorescent quantitative PCR,the percentages of U87MG cells in different cell cycles and the apoptotic rates in three groups were detected by flow cytometry,and the expressions levels of TRIM29, CyclinD1, p21, Bcl-2 and Bax proteins in the U87MG cells in three groups were detected Western blotting method. Results: There were significant differences in the expression levels of TRIM29, CyclinD1, P21, Bcl-2, Bax proteins, the expression levels of TRIM29 mRNA,and the percentages of U87MG cells in G₀/G₁, S, G₂/M phases and the apoptotic rates among three groups (P<0.05).Compared with control group, the expression levels of TRIM29 mRNA and TRIM29, CyclinD1, Bcl-2 proteins, the percentages of U87MG cells in G₀/G₁ phase, S phase,and G₂/M phase, and the apoptotic rates in the U87MG cells in NC-siRNA group had no significant differences (P>0.05).Compared with control group or NC-siRNA group,the expression levels of TRIM29 mRNA and TRIM29, CyclinD1,Bcl-2 proteins in the U87MG cells in TRIM29-siRNA group were decreased significantly(P<0.05); the percentage of U87MG cells in G₀/G₁ phase, the apoptotic rate of U87MG cells and the expression levels of p21 and Bax proteins were increased significantly (P<0.05). Conclusion: TRIM29-siRNA transfection can induce the cell cycle arrest and apoptosis in the U87MG cells and its mechanism may be related to down-regulation of Cyclin D1, Bcl-2 protein expressions and up-regulation of p21 and Bax protein expressions.

Key words: glioma, tripartitemotif containing 29, cell cycle, apoptosis

CLC Number:

R739.41

XIAO Hua, YAO Shengtao. Effects of TRIM29-siRNA transfection on cell cycle and apoptosis of glioma U87MG cells[J].Journal of Jilin University(Medicine Edition), 2020, 46(02): 323-328.

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Effects of TRIM29-siRNA transfection on cell cycle and apoptosis of glioma U87MG cells

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