Journal of Jilin University(Medicine Edition) ›› 2021, Vol. 47 ›› Issue (4): 1038-1042.doi: 10.13481/j.1671-587X.20210431

• Methodology • Previous Articles     Next Articles

Establishment of cultural method of primary SD rat aortic endothelial cells and their identification

Yuanyu TANG1(),Haiqin LIU2,Huagen MA3   

  1. 1.Teaching and Research Office of Basic Theory of Traditional Chinese Medicine,College of Traditional Chinese Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China
    2.College of Integrated Traditional Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China
    3.Teaching and Research Office of Jin Gui,College of Traditional Chinese Medicine,Beijing University of Traditional Chinese Medicine,Beijing 100029,China
  • Received:2020-11-25 Online:2021-07-28 Published:2021-07-22
  • Contact: Yuanyu TANG E-mail:2422198977@qq.com

Abstract: Objective

To establish a simple and efficient cultivating method of the primary SD rat aortic endothelial cells(AECs),and to provide an important carrier and tool cells for the exploration of the molecular biologial characteristics of AECs and the study of cardiovascular and cerebrovascular diseases.

Methods

One SD rat was selected, the chest was opened, and the aorta was removed in sterile condition; the connective tissue and fat outside the blood vessel were removed;the aortic tunica intima was everted and the aorta were cut into 1.0-1.5 mm vascular segments,and the vascular segments were placed in a culture bottle for primary culture. Then the vascular segments were removed 7 d later. The cultured cells were identified by morphological observation and immunocytochemical staining of factor Ⅷ(FⅧ) related antigen.

Results

After 3 d of culture, a small number of cells migrated from the vascular segments to form the island cell mass. From the 10th to 12th days, the adherent cells covered most of the culture bottle surface, and presented a typical “paving stone” mosaic arrangement. The results of immunocytochemical staining of FⅧ showed that the nucleus and the cytoplasm were brownish red,and the expression was positive; the positive cell rate was more than 98%.

Conclusion

Adherent culture method of aortic intima valgus can successfully isolate and culture the primary rat AECs.

Key words: aorta, vascular endothelial cells, intima valgus adherent culture method primary culture, immunocytochemical staining, rats,SD

CLC Number: 

  • R329.21